Bio-rad Nuvia™ S Media User Manual

Bio-Scale™ Nuvia™ S Cartridges1 and 5 mlInstruction ManualCatalog #732-4420 732-4421 732-4422 732-4423

62. Assemble with fittings and lock rings as shown in Figure 1 (use orange lock rings and medium size barb fittings with 1.6 mm tubing).Platen pressure

73. To maximize gradient accuracy and apply samples efficiently, install 1.6 mm ID tubing from the pump to the MV-6 sample inject valve (if available).

84. Connect the inlet of the cartridge to the male luer fitting on the MV-6 sample inject valve (Figure 2). If not using the MV-6 sample inject valve,

9Fig. 3. Column and fittings.Outlet fittingInlet fitting

10Section 3 Connecting to Other Liquid Chromatography SystemsBio-Scale™ Mini cartridges can be connected to any liquid chromatography system provided

113.1 BioLogic DuoFlow Systems The luer to BioLogic system fittings kit (catalog #732-0113) includes ¼–28 female to male and ¼–28 female to female luer

12with a new piece of stainless steel tubing attached to the union. Slip a short length of 0.8 mm ID tubing over 1/16 in OD stainless steel tubing to

13Section 4 Preparing a Cartridge for UseBio-Scale™ Mini Nuvia™ cartridges contain 20% ethanol + 0.1 M NaCl (v/v) as the shipping solution. The fully

143. Wash with degassed high-salt buffer for 5 min. 4. Equilibrate with low-salt buffer for 5 min. 5. Reduce the flow rate to the rate that will be us

15Table 3. Products for buffer exchange.Sample Volume Product MW Cutoff Catalog #50–100 µl Bio-Spin® 6 6 kD 732-6002 columns Bio-Spin 30 30

Bio-Rad Technical SupportFor help and technical advice, please contact the Bio-Rad Technical Support department. In the United States, the Technical S

16An appropriate starting point for purifying samples is a linear gradient of 0–0.4 M NaCl spanning 1–20 column volumes at 120 cm/hr, 0.5 ml/min for t

17At the end of each run, the cartridge can be regenerated with 1.0 M NaCl followed by starting buffer. Return to the desired flow rate and proceed wit

18Section 5 Cleaning-in-Place (CIP) and Sanitation If a column no longer yields reproducible results, the media may require thorough CIP and sanitati

192. To reequilibrate the column, wash it with 2–4 bed volumes of 0.5–2 M NaCl solution (containing 50–100 mM buffer). 3. If lipid removal is required

20Section 6 Ordering Information Bio-Scale™ Mini CartridgesFor a list of available cartridges*, visit www.bio-rad.com/cartridges/. Description 1 x

21Fittings Kits Catalog # Description 732-0111 Luer to M6 Adaptor Fittings Kit, includes luer to M6 fitting to connect 1 cartridge to an FPLC system

22731-8226 3.2 mm Barb to Male Luer, pkg of 25731-8225 1.6 mm Barb to Male Luer, pkg of 25731-8224 0.8 mm Barb to Male Luer, pkg of 25731-8223 3.2

23Section 7 Related Reading Harris ELV and Angal S (1989). Protein Purification Methods: A Practical Approach (Oxford: IRL Press). Scopes RK (1987). P

Life ScienceGroupBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385

ContentsSection 1 Introduction... 1Section 2 Connecting to Bio-Rad’s Low-Pressure Chromatography

Section 5 Cleaning-in-Place (CIP) and Sanitation...185.1 Autoclaving... 195.2 Storage...

1Section 1 IntroductionBio-Scale™ Mini cartridges have a patented* double-wall design that provides extra durability and allows easy, reliable runs w

2The design of the Bio-Scale Mini cartridges offers:n Convenience — the cartridge is ready for equilibration in the buffer of choice n Versatility —

3Table 1. Bio-Scale Mini Nuvia S cartridge specifications. Sizes 1 and 5 ml bed volumesDimensions 1 ml: 40 x 5.6 mm inner diameter (ID)

4Table 2. Nuvia S media specifications. Type of exchanger Strong cationFunctional group –SO3–Total ionic capacity 90–150 µeq/mlDynamic b

5Section 2 Connecting to Bio-Rad’s Low-Pressure Chromatography InstrumentsBio-Scale™ Mini cartridges are ideal for use with Bio-Rad’s BioLogic™ LP s