Bio-rad Microbial Culturing Module User Manual

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Microbial Culturing Kit
Catalog #166-5020EDU
explorer.bio-rad.com
Store components of this kit at room temperature.
For technical support, call your local Bio-Rad office or, in the U.S., call 1-800-424-6723
Duplication of any part of this document permitted for classroom use only.
Please visit explorer.bio-rad.com to access our selection of language
translations for Biotechnology Explorer™ kit curricula.
Biotechnology Explorer
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Summary of Contents

Page 1 - Microbial Culturing Kit

Microbial Culturing KitCatalog #166-5020EDUexplorer.bio-rad.comStore components of this kit at room temperature.For technical support, call your local

Page 2

6Streaking Plates with BacteriaStreaking is done to make single colonies from concentrated bacteria. Each colony grows fromone bacterium and thus a co

Page 3 - Table of Contents

7GlossaryAgar — Agar is a jelly-like substance obtained from seaweed. It is made of linked sugars (apolysaccharide) and is used to make medium for gro

Page 4 - Introduction

Life ScienceGroup00-0000 0000 Sig 0211Bulletin 0000 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Austral

Page 6 - Preparation of Liquid Media

Table of ContentsPageIntroduction...

Page 7 - Starting Minipreps

IntroductionThe microbial culturing kit provides the materials and methods for a wide variety of microbiology activities. The kit can be used to make

Page 8 - Preparation of Solid Media

Kit Inventory ChecklistKit Components Number/kit (✔)Ampicillin 2 vials ❐LB nutrient agar powder (to make 500 ml) 1 pouch ❐Petri dishes, sterile, bag o

Page 9 - Procedure for Pouring Plates

Preparation of Liquid MediaPreparation of AmpicillinAmpicillin is an antibiotic used to selectively eliminate bacteria that have not been transformedw

Page 10

Preparation of Ampicillin BrothThis protocol is used to prepare liquid LB broth with ampicillin which will select for the growth ofbacteria transforme

Page 11 - Glossary

Preparation of Solid MediaPreparation of AmpicillinAmpicillin is an antibiotic used to selectively eliminate bacteria that have not been transformedwi

Page 12 - 1665022 Rev A

Preparation of LB-Ampicillin Agar This protocol is used to prepare solid LB-ampicillin media which will select for the growth ofbacteria transformed w

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