BIO RAD 200/2.0 User Manual

Technical NoteUse of E-PAGE™48- and 96-Well Gels on theOdyssey®Infrared Imaging SystemsPublished October 2008. Revised October 2011. The most recent

I. IntroductionThe E-PAGE 48-and 96-well electrophoresis systems (Invitrogen Corporation, Carlsbad, CA) pro-vide a high-throughput SDS-PAGE method fo

Semi-Dry Transfer:• Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad, P/N 170-3940)• NuPAGE®Sample Reducing Agent (Invitrogen, P/N NP0009

10. At the end of the run, the E-Base will beep and the light will flash red. Press the pwr/prg but-ton to complete the run and stop the beeping/flas

11. While holding the tabs together, pull the stack assembly together through the rollers using one smooth motion until it reaches the Gel Barriers on

4. Carefully remove the trimmed gel from the cassette and place into a clean container with 200 mL of 1X transfer buffer (enough to fully submerge the

Use of E-PAGE™Gels on the Odyssey®Infrared Imaging System – Page 7Figure 1. E-PAGE 48-Well gel (left) loaded in duplicate with four different sets of

Figure 2. A. E-PAGE 96-Well gel loaded with replicate untreated or EGF-treated A431 cell extract sam-ples (alternating rows). After transfer with t