Bio-rad Prestained SDS-PAGE Standards User Manual download pdf

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

4006024 Rev D

Bio-Rad’s prestained standards are available

in high, low, and broad molecular weight

ranges. Blue dye has been covalently attached to

the standard proteins and will not be dissociated

by normal staining or destaining. The protein

mixtures are provided in a stable aqueous solu-

tion. No reconstitution or further dilution is

required before use.

Applications

Prestained SDS-PAGE Standards provide a

quick and easy way to assess the quality of an

electrophoretic transfer and act as a control in

repetitive blotting experiments. The visibility of

the standards makes it possible to monitor the

separation of proteins while electrophoresis is

in progress, even after the dye front has run off

the gel. The standards can also be used to locate

a protein for excision from an unstained prepar-

ative gel.

Specifications

Contents Approximately 625 µg total protein*

in 33% (v/v) glycerol, 3% SDS, 10

mM Tris pH 7.0, 10 mM DTT, 2 mM

EDTA, 0.01% NaN

Storage -20 °C

Shelf life 1 year at -20 °C

Volume 500 µl

Applications 25-100

per vial

* Total protein concentration is an approximation based

on the average concentrations of multiple lots. Actual

concentrations will vary.

Coomassie is a trademark of ICI.

Reference

1. Laemmli, U. K., Nature, 227, 680 (1970).

Ordering Information

Catalog

Number Product Description

Prestained Standards

161-0305 Prestained SDS-PAGE Standards, low range, 500 µl

161-0309 Prestained SDS-PAGE Standards, high range, 500 µl

161-0318 Prestained SDS-PAGE Standards, broad range, 500 µl

161-0324 Kaleidoscope Prestained Standards, 500 µl

161-0325 Kaleidoscope Polypeptide Standards, 500 µl

Molecular Weight Standards

161-0304 SDS-PAGE Standards, low range, 200 µl

161-0303 SDS-PAGE Standards, high range, 200 µl

161-0317 SDS-PAGE Standards, broad range, 200 µl

161-0314 Silver Stain SDS-PAGE Standards, low range, 200 µl

161-0315 Silver Stain SDS-PAGE Standards, high range, 200 µl

161-0306 Biotinylated SDS-PAGE Standards, low range, 250 µl

161-0311 Biotinylated SDS-PAGE Standards, high range, 250 µl

161-0319 Biotinylated SDS-PAGE Standards, broad range, 250 µl

161-0326 Polypeptide SDS-PAGE Standards, 200 µl

Specialty Standards

161-0310 IEF Standards, pI range 4.45-9.6, 250 µl

161-0320 2-D SDS-PAGE Standards, 500 µl

Prestained

SDS-PAGE Standards,

Low Range

Catalog Number

161-0305

Product shipped at room temperature.

Store at -20 °C upon arrival

4006024D 10/27/98 3:01 PM Page 1

1 2 3

Summary of Contents

Page 1 - Low Range

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 945474006024 Rev DBio-Rad’s prestained standards are availablein high, low, and broad molecu

Page 2

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 945474006024 Rev DBio-Rad’s prestained standards are availablein high, low, and broad molecu

Page 3 - Instructions for Use

Protein Molecular WeightsThe molecular weights of every lot ofprestained standards are individually calibratedagainst Bio-Rad’s SDS-PAGE Standards. Th

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Bio-rad Prestained SDS-PAGE Standards User Manual

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Summary of Contents

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