Bio-rad Profinity IMAC Resins User Manual download pdf (Page 2)

Table of Contents

Section 1 Introduction ..............................................................1

Section 2 Product Description ................................................1

Profinity

™

IMAC Resins and UNOsphere

™

Technology...1

Chemical Interactions ...................................................2

Resin Characteristics....................................................3

Chemical Compatibilities ..............................................4

Section 3 General IMAC Procedures .....................................5

Protein Binding.............................................................5

Washes ........................................................................5

Elution..........................................................................6

Purification Under Denaturing Conditions .....................6

Purification Under Nondenaturing Conditions ...............7

Imidazole Concentrations .............................................7

Section 4 Column Packing — Medium-Pressure

Columns ....................................................................9

Slurry Packing an IMAC Column...................................9

Recommended Columns..............................................9

Materials ......................................................................9

Resin Preparation.......................................................10

Method ......................................................................10

Section 5 Column Packing — Sample Preparation-Sized

Columns ..................................................................12

Materials ....................................................................12

Resin Preparation.......................................................12

Method ......................................................................12

Section 6 Immobilizing Metal Ions .......................................14

Section 7 Preparation of Clarified E. coli Lysate

Using Nondenaturing Conditions........................15

Materials ....................................................................15

Method ......................................................................16

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Bio-rad Profinity IMAC Resins User Manual Page 2