BIO RAD Bio-Scale User Manual

Bio-Scale™Mini Bio-Gel®P-6 DesaltingCartridges, 10 and 50 mlInstruction ManualCatalog #732-5304, 732-5312, 732-531410006995B.qxp 6/13/2007 9:00 AM

2. To apply samples efficiently, install 1.6 mm IDtubing from the pump to the MV-6 sampleinject valve (if available). If using the MV-6 sample inject

3. Connect the inlet of the cartridge to the maleluer fitting on the MV-6 sample inject valve (seeFigure 2). If not using the MV-6 sample injectvalve

Fig. 3. Cartridge and fittings.810006995B.qxp 6/13/2007 9:00 AM Page 8

Section 3Connecting to Other LiquidChromatography SystemsBio-Scale Mini cartridges can be connected to any liquidchromotography system, provieded that

Fig. 4. Luer to 1/4-28 adaptor.3.2 HPLC SystemsThe luer to 10-32 adaptor fittings kit (catalog #732-0112), provides fittings necessary to connectthe

3.3 FPLC SystemsThe luer to M6 adaptor fittings kit (catalog #732-0111) provides fittings necessary to connectthe Bio-Scale Mini cartridge to the M6

Section 4Preparing a Cartridge For UseBio-Scale Mini Bio-Gel P-6 cartridges contain 20 mM Bis-Tris, pH 6.5 + 0.05% sodium azide asthe storage solution

3. Equilibrate the cartridge with 2.2 column volumesof degassed equilibration buffer: 6 minutes at theroom temperature flow rate setting or 12 minutes

Section 5Desalting and BufferExchangeSamples can be desalted or the sample bufferexchanged by connecting the cartridge to a liquidchromatography syste

5.1 With a Liquid Chromatography System1. Set the flow rate to the desired flow.a. 10 ml cartridge: 0.5–4.0 ml/min at roomtemperature, 0.5–2.0 ml/mi

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Section 6Technical AssistanceFor additional information and technical assistance,contact your local Bio-Rad representative as listedon the back cover

Section 7Ordering InformationBio-Scale Mini Cartridges*Catalog #sDescription 5 x 1 ml 1 x 5 ml 5 x 5 mlUNOsphere™Q Support 732-4100 731-4102 731-4104

Fittings KitsCatalog # Description732-0111 Luer to M6 Adaptor Fittings Kit, includes luer toM6 fitting to connect to an FPLC system732-0112 Luer to

Section 8ReferencesGagnon P, Avoiding Instrument Associated Variationin Purification Scale-up and Scale-down, BioPharm,10 (3) 42–45, 1997Harris ELV an

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Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr.Hercules, CA 94547 USA510-741-10001-800-424-672310006995 Rev C10006995B.qxp 6/13/2007 9:00 AM P

Table of ContentsSection 1...Introduction ...1Section 2 Connecting to Bio-Rad’sLow-Pressure ChromatographyInstrum

Section 7 Ordering Information...17Section 8 References ...1910006995B.qxp 6/13/2007 9:00

Section 1IntroductionBio-Scale Mini cartridges have a patent-pendingdouble-wall design that provides extra durability andallows easy, reliable runs wi

• Luer fittings for convenient connection to anychromatography systemBio-Scale Mini Bio-Gel P-6 cartridges are packedwith Bio-Gel®P-6 gel. This gel co

Table 1. Bio-Scale Mini Bio-Gel P-6 cartridge specifications.Size 10 and 50 ml bed volumeDimensions 10 ml: 80 mm length x 12.6 mm innerdiameter50 m

Section 2Connecting to Bio-Rad’sLow-PressureChromatography InstrumentsBio-Scale Mini cartridges are ideal for use with Bio-Rad's BioLogic™LP chro

(Use orange lock rings and medium size barb fittings with 1.6 mm tubing.)Fig. 1. BioLogic LP system setup.1Alarm234567 809C .Platen pressurescrewLock