Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep User Manual download pdf (Page 21)

Table 4. Common buffers for ion exchange

chromatography.

Type of Buffering Buffer Range

Cation Exchanger

Acetic acid 4.8–5.2

Citric acid 4.2–5.2

HEPES 7.6–8.2

Lactic acid 3.6–4.3

MES 5.5–6.7

MOPS 6.5–7.9

Phosphate 6.7–7.6

PIPES 6.1–7.5

Pivalic acid 4.7–5.4

TES 7.2–7.8

Tricine 7.8–8.9

Anion Exchanger

Bicine 7.6–9.0

Bis-Tris 5.8–7.2

Diethanolamine 8.4–8.8

Diethylamine 9.5–11.5

L-Histidine 5.5–6.0

Imidazole 6.6–7.1

Pyridine 4.9–5.6

Tricine 7.8–8.9

Triethanolamine 7.3–8.0

Tris 7.5–8.0

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