BIO RAD 170-6558 Instruction Manual

INSTRUCTION MANUALCatalog Numbers170-6555170-6556170-6557170-6558170-6559GLYCO DOCGEL IMAGING SYSTEM

6Installation and Startup1.2.3 Installing the Glyco Doc SoftwareNoteIf your purchase included a computer, proceed to section 1.3. Your computerwas sh

7Installation and Startup1.3 VERIFY SYSTEM OPERATIONThis section provides a general description of the use of the Glyco Doc system.1.3.1 Starting the

8Installation and Startup1.3.2 Acquire an Image using the Test PlateTo verify system operation, start by acquiring an image of the Test Plate.NoteIf

9Installation and StartupTo acquire an image using the text plate, follow the procedure below.1. Turn on the Imager and set the Filter Switch to 500 n

10Installation and Startup1.4 IMAGE ANALYSIS USING THE IMAGE PROVIDED WITH YOUR SYSTEMThe key steps to analyzing an image are:• Open the image file (

11Installation and StartupFigure 7. Image Display, Control, and Status Windows5. Selectively insert and mark a band or remove a marker from a band of

12Installation and Startup7. Analyze band data as presented in the Band Tables. The following tables are available:Band Location DescriptionBand Loca

13Installation and Startup1.5 PREPARING FOR GEL IMAGINGThis section discusses the preparation of gels prior to imaging. The table below provides some

14Installation and Startup1.6 EXAMPLE OF A STANDARD LANE ANALYSISIn the oligosaccharide sample image (demo1.raw) provided with your Glyco Doc system,

15Software Reference2.0 SOFTWARE REFERENCEThis chapter discusses each of the software functions. The chapter is organized according to the software’s

TABLE OF CONTENTSSafetySection 1.0 Installation and Startup ...

16Software ReferenceDensitometer ReportsA printout is generated for each lane, with the Densitometer positioned next to its corresponding lane.All qua

17Software ReferenceImage FileTo export an image from Glyco Doc, so that it can be incorporated into a report or document created ina different applic

18Software Reference2.2 IMAGE MENUTo obtain the best image from which both image and band data files are derived, the Image menu offers anumber of set

19Software ReferenceThe Exposure Time is influenced by how intensely the fluorophore is emitting light. This, in turn, is pro-portional to the concen

20Software ReferenceAcquire OptionsThese selections must be made before you acquire the image. Select Image RectangleAfter you acquire the first imag

21Software Reference2.3 BANDS MENUGlyco Doc identifies bands by looking for sudden drop-offs in intensity. Glyco Doc then groups bandsaccording to th

22Software ReferenceInsert BandThis selection allows you to insert a band which may otherwise have been overlooked by the automaticband marking functi

23Software ReferenceSet Quantitive Standard Band...This allows you to select a band other than the standard.Display Standard Plot on Log ScaleThis all

24Software Reference2.4 OPTIONS MENUThe Options menu provides a number of display selection, each of which is discussed below.Reverse PaletteWhen sele

25Software Reference2.5 WINDOW MENUThis menu allows you to specify what type of data you want to display.Band TableA band table is a resizable and scr

Figures1. Glyco Doc Imaging System...12. Inst

26Software ReferenceRfRf (Relative mobility) are determined by setting the center point of one band to have an Rf = 1 and thatof another band to have

27Software ReferenceShow Selected LanesThis mode displays the intensity levels within a lane or compares between two or more lanes. Includethe first

28Theory of Operation3.0 THEORY OF OPERATIONSDuring exposure, the Imager’s CCD (Charge Coupled Device) detector collects photons emitted by the fluo-r

29Maintenance and Troubleshooting4.0 MAINTENANCE AND TROUBLESHOOTINGIn this chapter, the following Imager hardware maintenance procedures are given:•

30Maintenance and Troubleshooting8. Perform the previous steps in reverse to install the new tube and close-up the Imager.9. Create a new Bright Field

31Maintenance and Troubleshooting4.2 CLEANING THE EXCITATION FILTERThe Excitation Filter is located immediately above the gel holder and just below th

32Maintenance and Troubleshooting4.3 PREPARING GEL PLATESThe gel plates should be free of smudges, blotches, or any film on both sides. Clean off any

33Maintenance and Troubleshooting2. From the Image menu’s Acquire Options, select Make New Bright Field File.Follow the on-screen instructions, direct

34Maintenance and Troubleshooting4.5 IMAGER TROUBLESHOOTINGTry all appropriate actions in the chart below for the imaging problem you are experiencing

35Maintenance and TroubleshootingTable 3 (continued)Imager Troubleshooting Indication Problem ActionToo little or too much of the Incorrect Image Rec

SAFETYWarningDisconnect the Glyco Doc Imager before servicing. Refer servicing to Bio-Radservice personnel. This Glyco Doc Imager is certified to me

36Initialization FileAPPENDIX A. INITIALIZATION FILEThe GLYCODOC.INI initialization file defines the default options an application automatically sele

37Initialization FileA.3 COLORSColors are designated by their Red, Green, and Blue contents. Some common colors are:• Red 255 0 0• Green 0 255 0• B

38Initialization FileA.4 BAND DESCRIPTIONSThe value set in the Band Associations Dialog Box is the band association pixel distance. It determineswhet

39Initialization File[Monosaccharides]• KeyBand=GlcNAc• RF0Band=GlcNAc• RF1Band=GalNAc• BandNames=GlcNAc 50.00,Galactose 50.00,Glucose 50.00,Fucose 50

40Imager SpecificationsAPPENDIX B. IMAGER SPECIFICATIONSImaging Type: Fluorescence, TransmittanceDetector Type: CCD array, 753 x 244 pixelsLight Sourc

41Warranty and Ordering InformationAPPENDIX C. WARRANTY AND ORDERING INFORMATIONThe Glyco Doc Imaging System is warranted for 1 year against defects i

42Warranty and Ordering InformationWARRANTY INFORMATIONModel: ______________________________________________________________________Serial Number:____

DC1706555 Rev CDeclaration of ConformityBio-Rad Laboratories, Inc., 1000 Alfred Nobel Drive, Hercules, California,94547, U.S.A., declares that the pr

Bio-Rad Laboratories Main Office, 2000 Alfred Nobel Drive, Hercules, California 94547, Ph. (510) 741-1000, Fx. (510) 741-5800Also in: Reagents Park, A

1Installation and Startup1.0 INSTALLATION AND STARTUPThe Glyco Doc Imaging System simplifies analysis of small format carbohydrate electrophoresis gel

2Installation and Startup1.1 UNPACKINGCarefully inspect the shipping cartons for any damage which may have occurred in shipping. Severe dam-age to a

3Installation and StartupTo install the camera board, follow the procedure below.1. Turn off your computer and unplug the power cord.This procedure as

4Installation and Startup7. Place the Camera board in the slotThe connector on the Camera board must face the back of the computer. Insert the board,

5Installation and StartupTo set-up the Imager, you need a Phillips head screw driver. Refer to Figure 4.1. Locate the Filter Lock Screw on the bottom