Bio-rad Bio-Plex Manager™ MP Software Upgrade User Manual

Bio-Plex Manager™ MP SoftwareUser GuideVersion 1.0

1 | Introduction8 | Bio-Plex Manager MP SoftwareBio-Plex Data Pro is an optional software application that allows you to do further analysi

A | License Activation98 | Bio-Plex Manager MP Software4. Enter your activation code and click Deactivate.Note: The Activation Code field w

Deactivating the License through Technical SupportUser Guide | 9 9Deactivating the License through Technical SupportIf the computer on which Bio-P

A | License Activation100 | Bio-Plex Manager MP Software4. In the Export Deactivation Certificate window, click Save.A dialog box appears as

Deactivating the License through Technical SupportUser Guide | 101The File Successfully Saved window appears with instructions on how to send the

A | License Activation102 | Bio-Plex Manager MP Software

User Guide | 103B Backing Up the DatabaseThere are two databases that need to be backed up on a regular basis: xPONENT database Bio-Plex Manage

B | Backing Up the Database104 | Bio-Plex Manager MP SoftwareBacking up the xPONENT DatabaseBio-Rad recommends that you back up the xPONEN

Backing up the Bio-Plex Manager MP DatabaseUser Guide | 105You can specify the frequency with which you receive reminders to back up the database

B | Backing Up the Database106 | Bio-Plex Manager MP Software

User Guide | 107C TroubleshootingProblem Possible Cause SolutionInstrument is not connected. The connection to the MAGPIX instrument has been lost

User Guide | 92 Creating ProtocolsAbout ProtocolsA protocol contains the parameters of a Bio-Plex Manager™ MP software run. It includes informat

C | Troubleshooting108 | Bio-Plex Manager MP SoftwareLow Bead Count Three or more consecutive wells have bead counts lower than the recommen

User Guide | 109A Windows security alert dialog box appears with the message: Windows Firewall has blocked some features of this program.The user’

C | Troubleshooting110 | Bio-Plex Manager MP Software

User Guide | 111IndexAAnalytes, definition 12Analytes, excluding 12Analytes, selecting 13Analyzing results in Bio-Plex Manager MP 55Assay panel li

112 | Bio-Plex Manager MP SoftwareIndexFlat Bottom plate 27Fluidics Preparation routine 80Fluidics, checking status of 68IInstrument not connect

IndexUser Guide | 113Software activation, Product activation code 89Software deactivation 97Software deactivation, offline 99Software deactivation

114 | Bio-Plex Manager MP SoftwareIndex

Life ScienceGroup13-0782 0413 Sig 121210032257 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

2 | Creating Protocols10 | Bio-Plex Manager MP SoftwareNavigating to the Protocol Dialog BoxProtocols are created from the protocol dialog b

Creating a ProtocolUser Guide | 1 1Creating a ProtocolThe following are the steps to create a new protocol.To create a new protocol1. Click Create

2 | Creating Protocols12 | Bio-Plex Manager MP SoftwareAbout AnalytesBio-Plex Manager MP software groups analytes by panels. Preconfigured

Selecting AnalytesUser Guide | 1 3Selecting AnalytesAfter you select the assay panel you want to use in your experiment, you select the analytes y

2 | Creating Protocols14 | Bio-Plex Manager MP SoftwareFormatting PlatesThe array reader uses the plate formatting to identify which wells a

Formatting PlatesUser Guide | 1 5The template diagram in the Create/Run Protocols view displays the formatted wells in your protocol and the raw d

2 | Creating Protocols16 | Bio-Plex Manager MP SoftwareWell TypesThe following are different well types that you can specify on the plate: B

Formatting PlatesUser Guide | 1 7 Unknown WellsThe unknown wells contain samples of unknown concentration. These samples are taken from the subje

2 | Creating Protocols18 | Bio-Plex Manager MP SoftwareIn the following example, Autofill Down was used to create 20 Unknown wells. Note, th

Formatting PlatesUser Guide | 1 92. Click the Set Replicate Size dropdown list and pick the number of replicates in the group.Note: The maximum s

2 | Creating Protocols20 | Bio-Plex Manager MP SoftwareRemoving the Well FormattingRemoving the formatting of a well returns it to its unfor

Selecting a Standard LotUser Guide | 2 1Selecting a Standard LotBefore you can select the standard lot to use with your protocol, you must first s

2 | Creating Protocols22 | Bio-Plex Manager MP SoftwareOnly the standard lots that contain the selected analytes appear in the list. If the

Reusing a ProtocolUser Guide | 2 3Reusing a ProtocolYou can use a saved protocol as a template for your experiment. You can make changes to a copy

2 | Creating Protocols24 | Bio-Plex Manager MP Software

User Guide | 2 53 Running ProtocolsOnce your protocol is created, you are ready to run the protocol. The result is displayed in the raw data table

3 | Running Protocols26 | Bio-Plex Manager MP SoftwarePlate Handling GuidelinesBefore starting the run, note the following plate handling gu

Plate TypesUser Guide | 2 7Plate TypesThe plate height adjustment procedure adjusts the probe setting for four consumables, the well strip, and th

Bio-Plex Manager™ MP SoftwareUser GuideVersion 1.010032257 Rev A

3 | Running Protocols28 | Bio-Plex Manager MP SoftwareUsing the Plate HeaterFor some experiments, for example nucleic acid testing, you may

Running the ProtocolUser Guide | 2 9To run your protocol1. In the Create/Run Protocols view, click Start.The microplate platform automatically eje

3 | Running Protocols30 | Bio-Plex Manager MP SoftwareIf you select a pre-run routine, refer to the Reagent Block diagram and fill the appro

Running the ProtocolUser Guide | 3 1Reading the PlateIn the template diagram in the Create/Run Protocols view, the blue wells indicate which wells

3 | Running Protocols32 | Bio-Plex Manager MP SoftwareOnce the reading for a well is complete, the pulsing triangle resolves into one of the

Bead MapUser Guide | 3 3your analytes. The new analytes, if detected during the run, will then appear in the table.Column Heading DescriptionWell

3 | Running Protocols34 | Bio-Plex Manager MP SoftwareThe x-axis is the Classification 1 channel and the y-axis is the Classification 2 chan

Resuming a RunUser Guide | 3 5When a sample is being read, the Start button changes to a Stop button.Note: When you click Stop, Bio-Plex Manager

3 | Running Protocols36 | Bio-Plex Manager MP SoftwareEncountering and Resolving Low Bead CountsBio-Plex Manager MP notifies you when a lo

123 LEGEND1 Warning symbol indicating wells with a low bead count2 Warning symbol indicating region in which there is a bead count of fewer than 20 be

Bio-Rad Technical Support DepartmentThe Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Ti

3 | Running Protocols38 | Bio-Plex Manager MP SoftwareRun Results TableThe data from each run are saved as a result in the MAGPIX database.

Exporting Data to Bio-Plex Manager 6.xUser Guide | 3 9Exporting Data to Bio-Plex Manager 6.xBefore data can be analyzed in Bio-Plex Manager 6.

3 | Running Protocols40 | Bio-Plex Manager MP SoftwareExporting Data from the Create/Run Protocols ViewYou must first export the result to a

Exporting Data to Bio-Plex Manager 6.xUser Guide | 4 1Exporting Data from the Analyze/Export ViewThe result from a protocol reading is stored in t

3 | Running Protocols42 | Bio-Plex Manager MP SoftwareExporting Data Results Multiple TimesAs you analyze your data result in BPM 6.x, you m

User Guide | 4 34 Managing Assay Panels and Standard LotsIn Bio-Plex Manager™ MP software, you can select any of the Bio-Rad assay panels for us

4 | Managing Assay Panels and Standard Lots44 | Bio-Plex Manager MP SoftwareCreating Assay PanelsYou can create assay panels in the follow

Creating Assay PanelsUser Guide | 4 5Customizing an Existing Assay PanelBio-Plex Manager MP provides a list of all the assay panels it manufactu

4 | Managing Assay Panels and Standard Lots46 | Bio-Plex Manager MP SoftwareFrom the protocol dialog box, you can now select this new assa

Editing an Assay PanelUser Guide | 4 7Creating a New Assay PanelYou can create a new panel by starting with an empty assay panel and entering the

User Guide | iiiTable of ContentsChapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Chapter 2

4 | Managing Assay Panels and Standard Lots48 | Bio-Plex Manager MP Software Use the Add and Delete buttons to add or remove analytes fro

Restoring an Assay PanelUser Guide | 4 9To export an assay panel1. From the protocol dialog box, click Select Analytes.2. Click Manage Assay Panel

4 | Managing Assay Panels and Standard Lots50 | Bio-Plex Manager MP SoftwareA list of the predefined standard lot definitions and any stan

Creating Standard LotsUser Guide | 5 1Tip: Bio-Rad recommends you include the lot number of the standards. This will help you select the correct

4 | Managing Assay Panels and Standard Lots52 | Bio-Plex Manager MP SoftwareThe starting concentrations are specified on a label or produc

Editing Standard LotsUser Guide | 5 34. Enter a new name for the standard lot.5. Enter the expiration date for the standard lot.Tip: Click on th

4 | Managing Assay Panels and Standard Lots54 | Bio-Plex Manager MP Software3. In the Manage Standard Lots dialog box, select a standard l

User Guide | 5 55 Analyzing ResultsBio-Plex Manager™ MP is used to create and run your protocols. Once the plate has been read, you can review t

5 | Analyzing Results56 | Bio-Plex Manager MP Software In Bio-Plex Manager MP, there are no gated events. MAGPIX is a CCD, camera-based s

User Guide | 5 76 Maintaining the MAGPIX SystemThe Maintenance view is used to: Monitor the status of the MAGPIX instrument and to easily identif

iv | Bio-Plex Manager MP SoftwareTable of ContentsReading the Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

6 | Maintaining the MAGPIX System58 | Bio-Plex Manager MP SoftwareRecommended RoutineBio-Plex Manager™ MP software monitors the MAGPIX i

Daily Maintenance RoutinesUser Guide | 5 9Daily Maintenance RoutinesBio-Rad recommends that you run the following routines each day you use the MA

6 | Maintaining the MAGPIX System60 | Bio-Plex Manager MP SoftwareEnhanced MaintenanceThe Enhanced Maintenance routine will be recommended

Running a RoutineUser Guide | 6 1a. Click the Calibration Kits dropdown arrow to select the correct calibration kit.b. Click the Verification Kits

6 | Maintaining the MAGPIX System62 | Bio-Plex Manager MP SoftwareMonitoring the RoutineFrom the Maintenance view, you can monitor the sta

Monitoring the RoutineUser Guide | 6 3The following figure is an example of the Routine Commands dialog box. Progress bar The progress bar is a vi

6 | Maintaining the MAGPIX System64 | Bio-Plex Manager MP SoftwareSetting Up the Reagent BlockThe Reagent Block section of the Maintenance

LEGEND1The colored squares indicate which reservoirs to fill and specify the appropriate liquids.2The wells are color-coded to correspond to the reage

6 | Maintaining the MAGPIX System66 | Bio-Plex Manager MP SoftwareTo set up the Reagent Block1. Fill any colored reservoir with the indica

Calibration and Verification KitsUser Guide | 6 7Important: The reagents must be used with the calibration or verification values with the same l

User Guide | vTable of ContentsChapter 5 Analyzing Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55Chapter 6 Mainta

6 | Maintaining the MAGPIX System68 | Bio-Plex Manager MP SoftwareChecking the Status of the Instrument MaintenanceThe Status section of t

Responding to a Failed RoutineUser Guide | 6 9Status also shows the current status of the routine with the following symbols:Symbol DescriptionThi

6 | Maintaining the MAGPIX System70 | Bio-Plex Manager MP SoftwareCalibration FailuresIf the calibration routine fails, Bio-Rad recommends

Interrupting a RoutineUser Guide | 7 17. Run the Calibration and Verification routine. If verification fails a second time, contact Bio-Rad Techni

6 | Maintaining the MAGPIX System72 | Bio-Plex Manager MP SoftwareAdjusting the Probe HeightThe probe must be calibrated in order to work

MAGPIX MaintenanceUser Guide | 7 3Cleaning the Sample ProbeTo clean the sample probe1. Turn off the MAGPIX instrument and unplug the power cord.2.

6 | Maintaining the MAGPIX System74 | Bio-Plex Manager MP Software

User Guide | 7 57 Maintenance RoutinesThe maintenance routines keep the MAGPIX instrument in good working order and operating according to the man

7 | Maintenance Routines76 | Bio-Plex Manager MP SoftwareEstimated Run Time10 minutesTo Run the Calibration and Verification RoutineBefore y

Clear Bubbles RoutineUser Guide | 7 7Clear Bubbles RoutineDescription of RoutineThe Clear Bubbles routine performs a series of fluidics operations

vi | Bio-Plex Manager MP SoftwareTable of ContentsChapter 7 Maintenance Routines . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75Ca

7 | Maintenance Routines78 | Bio-Plex Manager MP SoftwareDaily Start Up RoutineDescription of RoutineDaily Start Up prepares your instrument

Enhanced Maintenance RoutineUser Guide | 7 9Enhanced Maintenance RoutineDescription of RoutineThe Enhanced Maintenance routine clears any bubbles

7 | Maintenance Routines80 | Bio-Plex Manager MP SoftwareFluidics Preparation RoutineDescription of RoutineThe Fluidics Preparation routine

Prepare for Storage RoutineUser Guide | 8 1Prepare for Storage RoutineDescription of RoutineThe Prepare for Storage routine clears all fluids from

7 | Maintenance Routines82 | Bio-Plex Manager MP SoftwarePrime RoutineDescription of RoutineThe Prime routine primes the fluidics system by

Shut Down RoutineUser Guide | 8 3Shut Down RoutineDescription of RoutineThe Shut Down routine cleans the fluidics lines and prevents a buildup of

7 | Maintenance Routines84 | Bio-Plex Manager MP SoftwareUnclog RoutineDescription of RoutineThis procedure performs a series of fluidics op

Verification RoutineUser Guide | 8 5Verification RoutineDescription of RoutineThe Verification routine ensures that the calibration settings are v

7 | Maintenance Routines86 | Bio-Plex Manager MP SoftwareWake from Storage RoutineDescription of RoutineThe Wake from Storage routine prepar

Wash Between Plates RoutineUser Guide | 8 7Wash Between Plates RoutineDescription of RoutineThe Wash Between Plates routine performs a series of f

User Guide | 71 IntroductionBio-Plex Manager™ MP is an application that allows you to run and maintain your MAGPIX instrument. MAGPIX monitors th

7 | Maintenance Routines88 | Bio-Plex Manager MP Software

User Guide | 8 9A License ActivationTo have access to all of the features of Bio-Plex Manager™ MP software, you must activate the product with a

A | License Activation90 | Bio-Plex Manager MP Software2. If the product license has not been activated, the Product Activation Required win

Activating the License through Technical SupportUser Guide | 9 1The Activation Successful window appears confirming that the software has been act

A | License Activation92 | Bio-Plex Manager MP SoftwareGenerating the Credentials FileTo generate the credentials file1. Start the Bio-Plex

Activating the License through Technical SupportUser Guide | 9 34. Select Send credentials to Technical Support and click Continue.5. Enter your a

A | License Activation94 | Bio-Plex Manager MP SoftwareThe File Successfully Saved window appears with instructions on how to send the crede

Activating the License through Technical SupportUser Guide | 9 5To activate Bio-Plex Manager MP with the license file1. Start the Bio-Plex Mana

A | License Activation96 | Bio-Plex Manager MP Software4. Click Activate.5. Navigate to where the license file is located, select the file,

Deactivating the Bio-Plex Manager MP LicenseUser Guide | 9 7Deactivating the Bio-Plex Manager MP LicenseYou may want to deactivate the Bio-Plex M