Bio-rad Bio-Plex Pro™ TGF-β Assays User Manual download pdf

This guide can be used to prepare and run a full 1 x 96-well assay plate. For

more information on a given step, refer to the complete instruction manual.

New users can download the manual at www.bio-rad.com/bio-plex.

Initial Preparation

1. Plan the plate layout.

2. Start up/warm up the Bio-Plex

system (30 min).

 n

Bring assay buffer, wash buffer, and sample diluent to room

temperature (RT). Keep other items on ice until needed

 n

Begin to thaw frozen samples

3. Prime wash station for ﬂat bottom plate or set vacuum manifold to

–1 to –3" Hg for ﬁlter plate.

4. Follow the prompts in Bio-Plex Manager

™

software to calibrate the

system. This can be done now or during an incubation step.

5. Mix 1 volume of Bio-Plex standard diluent with 3 volumes of Bio-Plex

sample diluent (each supplied in the kit). The resulting solution is used

for reconstitution and subsequent dilution of standards. This results in a

serum-matrix based diluent that mimics the matrix in 1:16 diluted serum

and plasma samples. For samples in serum-free media and other biological

ﬂuids, use a diluent that most closely matches the sample matrix. Add carrier

protein such as BSA at a ﬁnal concentration of 0.5% (w/v).

Bio-Plex Pro

™

Assays

Quick Guide 5

Important: Pay close attention to vortexing, shaking, and incubation instructions.

Deviation from the protocol may result in low assay signal and assay variability.

For use with Instruction Manual #

TGF-b Assays 10024984

1 2 3 4

Summary of Contents

Page 1 - Bio-Plex Pro

This guide can be used to prepare and run a full 1 x 96-well assay plate. For more information on a given step, refer to the complete instruction ma

Page 2

6. Reconstitute a single vial of standards in 500 µl of a diluent similar to the ﬁnal sample type or matrix as shown below. Vortex for 5 sec and in

Page 3 - Running the Assay

Running the Assay Note: Make sure all assay components are at RT before proceeding. 1. Prewet ﬁlter plate with 100 µl Bio-Plex assay buffer (skip f

Page 4 - Laboratories, Inc

13. Cover and incubate at 850 ± 50 rpm, as in Step 5, for 30 min at RT. 14. Wash the plate three times with 100 µl wash buffer.15. Resuspend beads i

Bio-rad Bio-Plex Pro™ TGF-β Assays User Manual

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Bio-rad Bio-Plex Pro™ TGF-β Assays User Manual

Browse online or download User Manual for Accessories for water Bio-rad Bio-Plex Pro™ TGF-β Assays. Bio-Rad Bio-Plex Pro™ TGF-β Assays User Manual

Summary of Contents

More documents for Accessories for water BIO RAD Bio-Plex Pro™ TGF-β Assays

Comments to this Manuals

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