Bio-rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual download pdf

This guide describes how to prepare and run a full 1 x 96-well ﬂat bottom

plate using a magnetic assay workﬂow. For more information on a given

step, refer to the corresponding section of the complete instruction manual.

New users can download the manual, which includes detailed instructions

and a list of kit components, at www.bio-rad.com/bio-plex.

Sample Preparation

1. Prepare lysates from cell culture or tissue samples. To remove insoluble

debris from lysates (≤2 ml), centrifuge at 15,000 x g for 10 min at 4˚C in

a benchtop microfuge. (Section 1 of the complete instruction manual)

2. Measure protein concentration using the Bio-Rad

™

protein assay.

3. Adjust protein concentration to 20–200 µg/ml in Bio-Plex

cell lysis buffer

containing cell lysis factor QG and PMSF. For samples with unknown

target expression or phosphorylation levels, start with 200 μg/ml protein.

4. If necessary, store lysates at –70˚C.

5. Thaw frozen lysates and keep on ice. Centrifuge again as above and

bring to room temperature (RT) immediately before use.

Bio-Plex Pro

™

Assays

Quick Guide 3

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.

Deviation from the protocol may result in low assay signal and assay variability. Do Not

mix phosphoprotein assays with corresponding total target assays (e.g., phospho-Akt

and total Akt). Use only the reagents supplied with cell signaling assay kits.

For Use With:

Product Family Instruction Manual #

Bio-Plex Pro cell signaling assays 10024929

1 2 3 4

Summary of Contents

Page 1 - Bio-Plex Pro

This guide describes how to prepare and run a full 1 x 96-well ﬂat bottom plate using a magnetic assay workﬂow. For more information on a given step

Page 2 - Assay — Day 1

Assay — Day 1 Note: Make sure all assay components are at RT before use. 1. Plan the plate layout. (Section 2 of the complete instruction manual)

Page 3

2. Prime wash station or prepare handheld magnetic washer. (Section 3) 3. Calibrate the Bio-Plex system by following the prompts within Bio-Plex

Page 4 - Laboratories, Inc

13. Resuspend beads in 125 μl bead resuspension buffer. Cover and shake at 1,100 rpm for 30 sec. 14. Read the plate using the appropriate settings a

Bio-rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual

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Bio-rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual

Browse online or download User Manual for Accessories for water Bio-rad Bio-Plex Pro™ Magnetic Cell Signaling Assays. Bio-Rad Bio-Plex Pro™ Magnetic Cell Signaling Assays User Manual

Summary of Contents

More documents for Accessories for water BIO RAD Bio-Plex Pro™ Magnetic Cell Signaling Assays

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