Bio-rad Apoptosis Assays User Manual download pdf

This guide can be used to prepare and run a full 1 x 96-well assay plate.

For more information on a given step, refer to the corresponding section

of the complete instruction manual. New users can download the manual,

which includes detailed instructions and a list of kit components, at

www.bio-rad.com/bio-plex.

A. Reagent Preparation

1. Reconstitute the following lyophilized reagents in dH

0 before use

according to the table below.

a. Allow vial to sit at room temperature for a minimum of 5 min, not to

exceed 30 min.

b. Mix by vortexing at a medium setting.

2. Bring the 10x assay buffer to ambient temperature (RT).

a. Mix by inversion to ensure all salts are into solution.

b. Prepare 1x assay buffer — dilute 1 part 10x assay buffer (60 ml) with

9 parts of dH

0 (540 ml).

Bio-Plex Pro

™

RBM Apoptosis Assays

Quick Guide

IMPORTANT! Pay close attention to vortexing, shaking, and incubation instructions.

Deviation from the protocol may result in low assay signal and assay variability.

For Use With Instruction Manual #

Bio-Plex Pro

™

RBM Apoptosis Assays 10033631

Reagent Volume, µl Reagent Volume, ml

Standards mix 150 Blocking buffer 1.5

Control 1 100 Standard diluent 1.0

Control 2 100 Detection antibodies 4.8

1 2 3 4

Summary of Contents

Page 1 - RBM Apoptosis Assays

This guide can be used to prepare and run a full 1 x 96-well assay plate. For more information on a given step, refer to the corresponding section

Page 2 - C. Sample Preparation

B. Dilution of Standard (1:3 Serial Dilution) 1. Label 8 polypropylene tubes S1 through S8. 2. Transfer the reconstituted standard into the tube l

Page 3 - D. Dispensing of Reagents

Bio-Plex Pro Assay Quick Guide 3. For sample analysis, bring samples to a ﬁnal protein concentration of 500 µg/ml by diluting with lysis dilution b

Page 4 - P10033632

10. Cover and incubate at 850 ± 50 rpm, as in Step 4, for 30 min at RT.11. Wash the plate three times with 100 µl 1x assay buffer. 12. After the ﬁn

Bio-rad Apoptosis Assays User Manual

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Bio-rad Apoptosis Assays User Manual

Browse online or download User Manual for Accessories for water Bio-rad Apoptosis Assays. Bio-Rad Apoptosis Assays User Manual

Summary of Contents

More documents for Accessories for water BIO RAD Apoptosis Assays

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