Bio-rad Bio-Gel P Polyacrylamide Gel User Manual

For technical support, call your local Bio-Rad office or, in the U.S., call 1-800-4BIORAD (1-800-424-6723)Profinia™Purificationand Buffer KitsInstruct

Fig. 3. Placement and positioning of denaturing IMAC buffers.After buffer bottle insertion, and the cartridge lines are automaticallyprimed, the cart

Table 4. Formulations for buffers and solutions provided in the GSTkits.Profinia Solution Supplied Concentrated Formulation Working 1x Formulation Vo

Fig. 4. Placement and positioning of GST buffers.After the buffer bottles are installed, the cartridges are ready to insertinto the instrument, and a

Bio-Gel®P-6 size exclusion resin, and typically yield ≥80% recovery ofapplied target protein. The resin in the Bio-Gel P-6 desalting cartridgesexhibit

conjunction with a desalting cartridge. A syringe and a 3-way stopcockcombination are used to fill the desalting loop. The desalting loop accessorycom

4. Return the 3-way stopcock lever to the middle position, facing towardthe syringe. The desalting loop is now filled with sample and ready forthe des

8. If the lysate is not going to be used immediately, it can be frozen at–20°C and thawed once to be purified at a later date. However, proteolysis or

6. If the lysate is not going to be used immediately, it can be frozen at–20°C and thawed once to be purified at a later date. See descriptionunder th

Section 7Frequently Asked Questions andTroubleshootingFor an extensive and comprehensive list of troubleshooting tips forprotein expression, sample pr

Leftover Buffer After Ten Runs• Each kit contains sufficient reagents for ten purification runs (equivalentto 10 ml of resin). Depending upon which me

Table of Contents...Section 1 Introduction...11.1 Background ...

Section 9Ordering InformationCatalog # DescriptionProfinia™ Systems620-1005 Profinia Instrument With Accessory Kit and Native IMACStarter Kit, 100–240

Catalog # Description620-0228 Profinia Desalting Purification Kit, 10 ml, includes Desaltingand Cartridge Cleaning Buffer Kit, 2 x 10 ml desalting car

Catalog # DescriptionBio-Scale™ Mini Affinity and Desalting Cartridges732-4610 Bio-Scale Mini Profinity IMAC Cartridges, 5 x 1 ml732-4612 Bio-Scale Mi

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Life ScienceGroup00-0000 0000 Sig 0308Bulletin 0000 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Australi

Section 1Introduction1.1 BackgroundThe Profinia™ protein purification system provides a complete line ofconsumables that interface with an automated,

2Table 1. Profinia purification and buffer kits.Kit Catalog # Solutions Powdered Reagent CartridgesPurification Desalting Storage Buffers Buffer Sol

1.4 Storage ConditionsAll unopened Profinia IMAC purification and buffer kits can be storedbetween 4 and 22°C. The GST kits can be stored at 22°C for

Table 2. Formulations for buffers and solutions provided in theNative IMAC Kits.Profinia Solution Supplied Concentrated Formulation Working 1x Formul

1. Replace buffer caps with buffer lids prior toinstallation into the instrument.2. For easy identification, buffer caps can beplaced directly above b

After placement of the buffer bottles and the cartridge lines have beenautomatically primed, the cartridges are ready to insert into the instrument.Th

Table 3 provides a list of denaturing IMAC buffer compositions. All buffersare 1x working buffer and placed on positions 1–7. The lysis buffer is used