Bio-rad DEAE Affi-Gel Blue Gel User Manual
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DEAE Affi-Gel®Blue GelInstruction ManualCatalog Number153-7307Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 945474006049 Rev A4006049A 8/
IntroductionDEAE Affi-Gel Blue gel is a bifunctional affinity/ionexchange chromatography matrix prepared by couplingCibacron®blue F3GA and diethylamin
Material Required but not SuppliedPre-wash buffer 0.1 M acetic acid, pH 3, 1.4 M NaCl,40% isopropanolRunning buffer see Table 2Regeneration buffer 2 M
5. Elute the column with three volumes of applicationbuffer.6. Apply the serum sample and elute with three bedvolumes of starting buffer. Collect frac
through desalting using the Bio-Gel P-6DG gel, orthrough dialysis. The buffers listed in Table 1 haveprovided complete plasminogen removal when tested
improved recovery of IgG, use Affi-Gel Protein Asupport.For more information and applications, requestBulletin 1092.CatalogNumber Product Description1
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