Bio-rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridg User Manual

UNOsphere SUPrA™Affinity Chromatography Media Instruction ManualCatalog #s156-0218156-0219156-0220156-0221156-0222156-0250Please read these instructio

6• Allow the slurry to settle until a 2–5 cm layer of clear supernatant packingbuffer is observed above the settled bed• Lower the top piston into sup

7 To obtain comparable HETP values between columns, the same conditions mustbe applied. The number of theoretical plates is often expressed in terms o

8Section 6Screening ConditionsBecause different antibodies will have differing levels of affinity for UNOsphereSUPrA™ media, it is highly recommended

9Section 7Operation and MaintenanceUNOsphere SUPrA™ media are designed to achieve high productivity, usability andscalability, to allow users to proce

10Section 9SanitizationIf microbial contamination of the packed bed is suspected, the column can beperiodically washed with a solution consisting of 0

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Table of ContentsSection 1 Introduction ...1Section 2 Technical Description ...

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1Section 1IntroductionUNOsphere SUPrA™ media are affinity chromatography support based onrecombinant protein A. The media are designed for process-sca

2Section 2Technical DescriptionComposition Highly Crosslinked PolymerParticle Size Range 53–61 µmLigand Recombinant Protein ACoupling Chemistry Epox

3Fig. 1. Flow performance of UNOsphere SUPrA™ media in Bio-Rad InPlace™ column (20 cm x 20 cm) packed to 13% axial compression.Section 3Preparation fo

43.1 Determining Slurry Percentage or Media VolumeThe recommended slurry percentage for column packing is 30–50%. There areseveral methods that can b

5• Carefully transfer the slurry to the column (using the pre calculated volume).Slurry can be transferred using a diaphragm pump or other gentle tran