Bio-rad Nuvia™ HR-S Media User Manual

Nuvia™ HR-SStrong CationExchange MediaInstruction ManualCatalog numbers156-0511156-0513156-0515156-0517 Please read these instructions before you use

6 Nuvia HR-S Instruction Manual Section 5: Column Packing EvaluationWhen column packing is complete, equilibrate the column until baseline conductiv

Nuvia HR-S Instruction Manual 7Section 6: Operation and MaintenanceA linear flow rate of 100 cm/hr with a 20 cm bed is a recommended starting poin

8 Nuvia HR-S Instruction Manual All buffers commonly used for ion exchange chromatography can be used with Nuvia media (see Table 2). Buffering ions

Nuvia HR-S Instruction Manual 9Section 7: Regeneration and SanitizationAfter each run, the packed bed should be washed with 2–6 bed volumes of 1–

10 Nuvia HR-S Instruction Manual Section 11: Ordering InformationCatalog # Description156-0511 Nuvia HR-S Media, 25 ml156-0513 Nuvia HR-S Media, 100

Life ScienceGroupSig 121210033316 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austri

Table of ContentsSection 1: Introduction ... 1Section 2 : Technical Description ...

Nuvia HR-S Instruction Manual 1Section 1: IntroductionNuvia™ HR-S cation exchange media is a new high-resolution chromatography media designed fo

2 Nuvia HR-S Instruction Manual Section 2: Technical DescriptionTable 1. Characteristics of Nuvia HR-S media. Nuvia HR-SType of ion exchanger Str

Nuvia HR-S Instruction Manual 3Section 3: PreparationNuvia HR-S media is supplied fully hydrated in 20% ethanol + 1 M NaCI as a 50% (v/v) slurry.

4 Nuvia HR-S Instruction Manual Section 4: Column PackingNuvia HR-S media can be packed using most conventional packing methods. A slurry concentrat

Nuvia HR-S Instruction Manual 5Packing Process-Scale ColumnsAfter removing the storage buffer (Section 3), prepare a 20–50% slurry (v/v) with pac