Bio-rad Bio-Gel P Polyacrylamide Gel User Manual

Bio-Gel®PPolyacrylamide GelInstruction ManualLIT174B 10/13/98 9:26 AM Page i

and myoglobin are visible and can be seen as they migrate through thecolumn.Section 6Sanitation and SterilizationBio-Gel P gel can be sterilized withi

17Section 8Flow Rate DeterminationGel filtration is a diffusion controlled process: the efficiency ofresolution depends on flow rate and gel bead size

19Section 9Ordering InformationCatalogNumber Product Description Comments150-4114 Bio-Gel P-2 Gel, Fine, 100 g Rapid carbohydrate and small peptide se

21CatalogNumber Product Description Comments150-4150 Bio-Gel P-30 Gel, Medium, 100 g Purification of proteins and polypeptides. Fractionation range of

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT174 Rev BLIT174B 10/13/98 9:27 AM Page 22

Table of ContentsSection 1 Introduction...1Section 2 Technical Description ...

Section 1IntroductionBio-Gel P gels are porous polyacrylamide beads prepared bycopolymerization of acrylamide and N,N'-methylene-bis-acrylamide.T

Section 2Technical DescriptionTable 1. Bio-Gel P Gel Product DescriptionMatrix Bio-Gel polyacrylamide gelParticle sizeMedium 90-180 µm Fine 45-90 µm E

5Table 2. Properties of Bio-Gel P-GelsTypicalTypical FractionationParticle Size Hydrated Bed Range/NominalRange, Hydrated Volume, ml/g Typical Flow Ex

Bio-Gel P gel is compatible with solubilizing and denaturing con-ditions used in molecular weight determinations such as 6 M guani-dine-HCl, chaotropi

2. Allow Bio-Gel P-2 through P-10 gels to hydrate 4 hours at roomtemperature (1 hour if buffer was previously brought to 100 °Cand then allowed to coo

6. Pour the even slurry into the column in a single, smooth move-ment. Avoid splashing the slurry, to insure even packing, and toavoid trapping air bu

sible to filter it, the sample should be centrifuged until it is clear.Figure 7 shows the hypothetical effects of various chromatographicconditions.13