Bio-rad Bio-Beads SM-2 Adsorbents User Manual

Bio-Beads®SM Hydrophobicand Polar InteractionAdsorbentsInstruction ManualBio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT195 Rev BLI

Table of ContentsSection 1 Introduction ...1Section 2 Product Description...2Section

Section 1IntroductionBio-Beads SM adsorbents, for adsorbing organicsfrom aqueous solutions, are neutral, macroporouspolymeric Analytical Grade adsorbe

detergents such as deoxycholate,4-6NP-40,7and emulgen911.8-10Bio-Beads adsorbents have been used to separatewater soluble steroids,11,12phenols,13drug

slurry is necessary to fill the column. Use wetdensity information from Table 1 to calculate thequantity of adsorbent needed for size of the column.2.

Section 4Sample Protocols4.1 Triton X-100 Detergent RemovalTriton X-100 detergent is useful for membranesolubilization. However, the usefulness of Tri

2. Incubate the beads with the solution at roomtemperature on a rotary platform to insure thoroughmixing. Stir for 30 minutes.3. Centrifuge the mixtur

8. Gibson, G. G. and Schenkman, J. B., J. Biol. Chem., 253,5957 (1978).9. Warner, M., J. Biol. Chem., 257 (21), 12995 (1982).10. Bruch, R. C., Thotaku

Section 8Product InformationCatalogNumber Product Description152-3920 Bio-Beads SM-2 Adsorbent, 20–50 mesh, 100 g152-8920 Biotechnology Grade Bio-Bead