Bio-rad DEAE Affi-Gel Blue Gel User Manual
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Econo-Pac®Serum IgGPurification Kit andEcono-Pac Serum IgGPurification ColumnsInstruction ManualCatalog Numbers 732-3037 and 732-2026For Technical Ser
Table of ContentsSection 1 Econo-Pac Serum IgG Purification Kit... 11.1 Introduction ...
Section 1Econo-Pac Serum IgG Purification KitCatalog Number 732-30371.1 IntroductionThe Econo-Pac serum IgG purification kit provides everything neces
1.4 Buffer PreparationHuman Application Buffer PreparationThe human buffer is supplied as a premixed, preweighed solid. Reconstitutionand filtration a
5. Allow the sample to completely run into the column. Discard the first 3.0 mleluted.6. Add 4.0 ml of the application buffer to elute the serum, whil
2. What is the purpose of the regeneration buffer wash the first time thecolumn is used?For new preparations of gel, this initial wash will elute resi
Application BuffersBuffer ionic strength is a critical parameter in DEAE Affi-Gel blue chro-matography. Small variations in salt concentration and pH
5.Elute the IgG with 20 ml of application buffer. For a more precise collectionmethod, collect fractions of volume approximately equivalent to that of
U.S. (800) 4BIORAD • California Ph. (510) 741-1000 • New York Ph. (516) 756-2575 • Australia Ph. 02-805-5000 • Austria Ph. (1) 877 89 01 • Belgium
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