Bio-rad Affi-Gel Protein A Media User Manual

Econo-Pac®Protein A KitProtein AColumnsInstructionManualCatalog Number732-2020732-2022For Technical ServiceCall Your Local Bio-Rad Office orin the U.S

Section 3References1. Ey, P. L., Prowse, S. J. and Jenkins, C. R., Immunochemistry, 15, 429 (1978).2. Begbee, W. L., Vanderlaan, M., Fong, S. S. N. an

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Table of ContentsSection 1 Econo-Pac Protein A Kit...11.1 Introduction ...

Section 1Econo-Pac Protein A Kit1.1 IntroductionThe Econo-Pac protein A kit is based upon Bio-Rad’s Affi-Gel®protein A MAPS®II methods and contins ev

1.3 Additional Items RequiredColumn rack The Econo-Pac 10 (12 place) acrylic rack isideal for holding the Econo-Pac columns.Other benchtop or lattice

1.5 Sample PreparationSample preparation is simplified by using the Econo-Pac 10DGcolumns. Each Econo-Pac 10DG desalting column can process up to3 mi

4. Wash the column with 20 ml binding buffer.5. Elute the IgG with 10 ml of elution buffer. Elute the columnwith an additional 20 ml of buffer to insu

4. Shelf life to the Econo-Pac protein A kit:The shelf life of the Econo-Pac protein A column is 1 year at4 °C. The unreconstituted buffers are good f

pleted with only one column volume of buffer per step, making thetotal procedure fast and easy.2.2 Buffer ConsiderationsThe affinity of IgG for prote

5. Elute the IgG with 10 ml of elution buffer. Elute the columnwith an additional 20 ml of buffer to insure total removal ofIgG. In most cases, the ma