Bio-rad Bio-Scale™ Mini Affi-Prep® Protein A Cartridges User Manual
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Affi-Prep®Protein A MAPS®II KitInstruction ManualLIT79B 9/1/98 8:12 AM Page A
Table of ContentsSection 1 Introduction... 11.1 P
Section 1IntroductionProtein A chromatography is one of the most reliable and specific methods for immuno-globulin purification. The Affi-Prep protein A
Fig. 1. Binding capacities for murine IgG subclasses. Mouse ascites fluids, containingequivalent amounts (5 mg) of either mouse IgG1, IgG2a, or IgG2bm
Section 2Instructions for Use2.1 Buffer PreparationBinding Buffer PreparationThe binding buffer is supplied as a premixed, preweighed solid. Dissolve
Note: For many murine IgGs the capacity is as high as 10 mg antibody/ml Affi-Prep proteinA or 5 mg antibody/Affi-Prep protein A analytical cartridge.5.
The shelf life of Affi-Prep protein A matrix is at least 1 year at 4 °C. The Affi-Prep MAPS IIbuffer solids are stable for at least 1 year when stored a
LIT79 Rev BEastern Regional Office, 85A Marcus Dr., Melville, New York 11747 • Phone (516) 756-2575 • Fax (516) 756-2594European Headquarters, Bio-Rad
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