Bio-rad CM Affi-Gel Blue Gel User Manual
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CM Affi-Gel®Blue GelInstruction ManualCatalog Number153-7304LIT308C 9/3/98 7:13 AM Page i
IntroductionCM Affi-Gel blue gel is a bifunctional affinity/ion exchangechromatography matrix prepared by coupling Cibacron blueF3GA and carboxymethyl
Material required but not suppliedPre-wash buffer 0.1 M acetic acid, pH 3, 1.4 M NaCl,40% isopropanolRunning buffer 10 mM K2HPO4, pH 7.25, 0.15 MNaCl,
8. Some loss of capacity due to small amounts of proteinwhich remain bound to the gel may be evident after aboutfive cycles. To compensate for this,
Instructions for a Protease FreeGlobulin Fraction From Serum1. Follow the general instructions through step 5.2. Measure the total volume of the col
9References1. Hudson, L. and Hay, F. C., Practical Immunology, p. 152,Blackwell Scientific Publications, Oxford, England (1976).2. Steinbuch, M., Au
Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547LIT308 Rev CLIT308C 9/3/98 7:13 AM Page 10
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