Bio-rad UNOsphere™ Rapid S Media User Manual

Bio-Scale™ Mini UNOsphere™ Q, S and Rapid SCartridges, 1 and 5 mlInstruction ManualCatalog #732-4100, 732-4102, 732-4400,732-4104, 732-4110, 732-4401,

(Use orange lock rings and medium size barb fittings with 1.6 mm tubing.)Fig. 1. Biologic LP setup61Alarm2 34567 809C .Platen pressurescrewLock ringT

2. To maximize gradient accuracy and apply samples efficiently, install 1.6 mm ID tubing fromthe pump to the MV-6 sample inject valve (ifavailable). I

3. Connect the inlet of the cartridge to the maleLuer fitting on the MV-6 sample inject valve. (SeeFigure 2.) If not using the MV-6 sample injectvalve

9Fig. 3. Column and fittings.Inlet fittingOutlet fitting10005454D:10005454A.qxd 9/24/2008 10:00 AM Page 9

Section 3Connecting to Other LiquidChromatography SystemsThe Bio-Scale™ Mini cartridges can be connectedto any liquid chromatography system, provided

3.1 BioLogic DuoFlow SystemsThe Bio-Scale Mini cartridge to BioLogic system fittings kit (catalog #732-0113), includes ¼–28female to male Luer and ¼–

3.3 FPLC SystemsThe Luer to M6 adaptor fittings kit (catalog #732-0111) provides fittings necessary to connectthe cartridge to the M6 fittings found o

Section 4Preparing a Cartridge ForUseBio-Scale™ Mini UNOsphere™ cartridges contain20% ethanol v/v as the storage solution. The fullyhydrated support i

4. Equilibrate the cartridge with low-salt buffer for 5 min.5. Reduce the flow rate to the rate that will be usedin the purification protocol. 4.1 Sam

Table 1 Products for Buffer Exchange.Sample RecommendedVolume Product Use Catalog #50–100 µl Bio-Spin 6 column Desalting proteins 732-60006 kD50–1

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Common buffers for cation exchange chromatographyare listed in Table 3.An appropriate starting point for purifying samples isa linear gradient from 0–

amounts, from 25 ml bottles to bulk quantities forscaling-up methods developed using the cartridges. UNOsphere Q, S, and Rapid S mediaare fully suppor

Table 3 Common Buffers for Ion ExchangeChromatography. 1,2,3Type of BufferingCation Ion Exchanger Buffer RangeAcetic acid 4.8–5.2Citric acid 4.2–5.2

Section 5 Cleaning-in-Place (CIP) andSanitationIf a column no longer yields reproducible results, themedia may require thorough CIP and sanitation tor

3. If lipid removal is required, the column may bewashed with a 20–50% ethanol solution at 50 cm/hr.5.1 AutoclavingBio-Scale Mini®cartridges are not a

Section 6Technical AssistanceFor additional information and technical assistance,contact your local Bio-Rad representative as listedon the back cover

Section 7Ordering InformationBio-Scale™ Mini Cartridges*Description 5 x 1 ml 1 x 5 ml 5 x 5 mlUNOsphere™Q Support 732-4100 731-4102 731-4104UNOsphere

Fittings KitsCatalog # Description732-0111 Luer to M6 Adaptor Fittings Kit, includes Luer to M6 fitting to connect to an FPLC system732-0112 Luer to 1

Section 8References 1. Harris ELV and Angal S, Protein PurificationMethods: A Practical Approach, IRL Press,Oxford (1989)2. Scopes RK, Protein Purific

FPLC is a trademark of GE Healthcare Group Companies. Luer-Lokis trademark of Becton, Dickinson and Co.Triton is a trademark of UnionCarbide Corp. Upc

Table of ContentsSection 1...Introduction. ...1Section 2...Connection to Bio-Rad’s Low-...Pressure Chro

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Life ScienceGroup00-0000 0000 Sig 0308Bulletin 0000 Rev A US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Australi

Section 6 ..Technical Assistance ...21Section 7 ..Ordering Information...22Section 8 ..References ...

Section 1IntroductionBio-Scale™ Mini cartridges have a patent-pending,double-wall design that provides extra durability andallows easy, reliable runs

• Ready-to-go convenience; simply equilibrate thecartridge in the buffer of choice• Luer fittings for convenient connection to anychromatography syste

Table 1 Bio-Scale Mini UNOsphere Q, S, &Rapid S Cartridge Specifications.Sizes 1 and 5 ml bed volumesDimensions 1 ml: 40 x 5.6 mm inner diamete

Table 2 UNOsphere Q, S, and rapid SSpecificationsUNOsphere Q UNOsphere S UNOsphere Rapid SType of ion exchanger Strong anion Strong cation Strong cat

Section 2Connecting to Bio-Rad'sLow-PressureChromatographyInstrumentsThe Bio-Scale™ Mini cartridges are ideal for use withBio-Rad's BioLogic