BIO RAD Bio-Plex Pro™ TGF-β Assays manuals

Owner’s manuals and user’s guides for Accessories for water BIO RAD Bio-Plex Pro™ TGF-β Assays.
We providing 2 pdf manuals BIO RAD Bio-Plex Pro™ TGF-β Assays for download free by document types: User Manual

Bio rad Bio-Plex Pro™ TGF-β Assays User Manual (43 pages)

Brand: Bio-rad | Category: Accessories for water | Size: 0.78 MB |

English

Table of contents

Bio-Plex Pro

1

TGF-b Assays

1

Table of Contents

2

Introduction

3

Principle

4

Kit Contents and Storage

6

Assay Workﬂow

9

Instruments and Software

10

Plan Plate Layout

11

2. Prepare Instrument

12

3. Prepare Wash Method

13

4. Prepare Standards

14

5. Prepare Samples

17

Lysates

18

Cell Culture Supernatant

18

6. Prepare Coupled Beads

20

7. Run Assay

22

96 60 5,940 6,000

25

48 30 2,970 3,000

25

8. Read Plate

27

Analyte Bead Region

28

TGF-b1 13

28

TGF-b2 72

28

TGF-b3 66

28

Fig. 4. Plate formatting

29

Acquire Data

30

Reacquire Data

31

Luminex xPONENT Software

32

Troubleshooting Guide

33

High Intra-Assay CV

34

Low Bead Count

35

High Background Signal

36

Poor Recovery

36

Impact of Sample Matrix

37

Plate Layout Template

38

Calculation Worksheet

39

Safety Considerations

41

Legal Notices

41

Ordering Information

42

Life Science

43

Bio-Rad

43

Laboratories, Inc

43

Bio rad Bio-Plex Pro™ TGF-β Assays User Manual (4 pages)

Brand: Bio-rad | Category: Accessories for water | Size: 0.36 MB |

English

Table of contents

Bio-Plex Pro

1

Running the Assay

3

Life Science

4

Bio-Rad

4

Laboratories, Inc

4

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