Bio-rad Fluorescent Protein Stains User Manual

Flamingo™Fluorescent Gel StainInstruction ManualCatalog Numbers161-0490, 20 ml161-0491, 100 ml161-0492, 500 mlFor technical support call your local Bi

83.2. Sensitivity with UV TransilluminationWhen scanned on the VersaDoc 4000 imaging system, an SDS-PAGE gel stainedwith Flamingo fluorescent gel stai

9R2 = 0.994305001000150020002500012345fluorescenceR2 = 0.994705000100001500020000250000 10203040506070ng β-galactosidasefluorescenceR2 = 0.99600500001

Section 4TroubleshootingProblem Possible Cause RemedyBands or spots not No protein on gel Verify that there is actuallyvisible protein on the gel byst

Problem Possible Cause RemedySpeckles or blotches in the Particulate material from Filter water used to preparegel image water, reagents, staining fix

Section 5Product Information4.1. Flamingo Fluorescent Gel StainCatalog # Description161-0490 Flamingo Fluorescent Gel Stain, 10x Solution, 20 ml161-04

10003321 Rev ALife ScienceGroup00-0000 0000 Sig 1204Bulletin 0000 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA (800)

Table of ContentsSection 1 Introduction and General Information...11.1 Introduction...

1Section 1Introduction and General Information1.1 IntroductionFlamingo fluorescent gel stain allows sensitive fluorescence visualization of proteins t

21.3 StorageThe product is stable for at least 6 months when stored at 2–8°C. Avoid exposureto temperatures greater than 37°C and protect from light.1

3020406080100250 300 350 400 450 500 550 600Excitation w avelength (nm)Fluorescence intensity %020406080100450 500 550 600 650Emission wavelength (nm)

4Section 2Instructions2.1 General ConsiderationsBest results are obtained using clean technique. Any dust or dirt transferred to thesurface of the gel

52.3 Fix GelsRemove the gel(s) from the gel cassette or plates. Place gel in a clean glass traywith the volume of fix solution indicated above. Cover

62.6 Background Reduction (Optional)Note: The dye in Flamingo fluorescent gel stain has very low fluorescence whennot protein-bound. A background redu

7Section 3Technical Information3.1. Sensitivity with Laser ScanningWhen scanned on the Molecular Imager FX system, an SDS-PAGE gel stainedwith Flaming