Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual

Trans-Blot®SDDNA/RNABlotting KitInstructionManualCatalog Number170-3957For use with the Trans-Blot SDsem-dry electrophoretic transfercell (catalog num

Section 7Equipment and AccessoriesKit Components161-0733 Tris/Boric Acid/EDTA, 1 L 170-3960 Extra Thick Blot Paper, 15 x 20 cm, 30Gel Support FrameTra

Section 8References1. Southern, E. M., Detection of specific sequences among DNA fragments separated by gel elec-trophoresis, J. Mol. Biol., 98, 503-5

Life ScienceGroupBio-Rad Laboratories Main OfficeAlso in: AustraliaAustriaPh. (1)-877 89 01, Fx. (1) 876 56 29 Belgium Ph. 09-385 55 11, Fx. 09-385 6

Table of ContentsPageSection 1 Introduction ... 11.1 S

NoteTo insure best results from the Trans-Blot SD DNA/RNA Blotting Kit, become fully acquaintedwith its instructions before using the kit. It is also

Section 1IntroductionThe standard capillary transfer of DNA/RNA from agarose gels to blotting membranes isusually an overnight procedure.1-3The Trans-

2Section 2Preparation for DNA Blotting1. Prepare enough 0.5x TBE transfer buffer for use in both the horizontal agarose gelelectrophoresis and the sem

Section 4General Assembly of Unit for Transfer1. Saturate two pieces of precut Extra Thick Blot Paper (provided with the kit) and precutZeta-Probe®GT

7. Place the safety cover onto the unit and plug the Trans-Blot SD cell into the power sup-ply. Be sure to maintain normal polarity of the electrodes,

b. Power conditions during transfer may have changed. It is important to have constantcurrent during the course of the run. If the buffer is less conc

Section 6Appendix10x TBE (per liter*), pH 8.3Quantity Final 0.5x Concentration108 g Tris base 44.5 mM Tris base55 g boric acid 44.5 mM boric acid40 m