Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual download pdf (Page 9)

Section 6

Appendix

10x TBE (per liter*), pH 8.3

Quantity Final 0.5x Concentration

108 g Tris base 44.5 mM Tris base

55 g boric acid 44.5 mM boric acid

40 ml 0.5 M EDTA, pH 8.0 1 mM EDTA

To make a 0.5x TBE working buffer, mix 50 ml of the 10x TBE stock per 1 liter of deion-

ized, distilled water.

* One liter of Bio-Rad premixed 10x TBE is included with the kit.

1x MOPS, 1 L

Quantity Final 1x Concentration

4.186 g MOPS 20 mM 3- (N- Morpholino) propanesulfonic acid, pH 7.0

0.41 g sodium acetate 5 mM sodium acetate

2 µl of 0.5 M EDTA, pH 8.0 1 mM EDTA

Dissolve 4.186 g MOPS, 0.41 g sodium acetate, and 2 µl EDTA in 800 ml of ddH

adjust volume to l liter with ddH

37% formaldehyde stock solution

1.8% formaldehyde - 5 ml of 37% stock/ 100 ml

6.5% formaldehyde - 1.75 µl of 37% stock/ 10 µl

20x SSC, 1 L

Quantity Final 20x Concentration

175.3 g NaCl 3 M NaCl

88.2 g sodium citrate 0.3 M sodium citrate

Adjust pH to 7.0 and bring volume to 1 liter with deionized, distilled water. Dilute the 20x

stock solution accordingly to make a 2x working solution.

Gel loading buffer - Bromophenol blue dye

1mM EDTA, pH 8.8

0.25% bromophenol blue

0.25% xylene cyanol

50% glycerol

1 2 3 4 5 6 7 8 9 10 11 12

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Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual Page 9