Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual download pdf (Page 4)

Section 1

Introduction

The standard capillary transfer of DNA/RNA from agarose gels to blotting membranes is

usually an overnight procedure.

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The Trans-Blot SD DNA/RNA blotting kit, used with the

Trans-Blot SD semi-dry transfer cell, allows blotting of DNA and RNA from agarose gels to

nylon membrane in only minutes, without any gel pretreatments. The Trans-Blot SD semi-dry

electrophoretic transfer cell generates a high field strength (V/cm

) which facilitates rapid

transfers. The blotting kit produces highly efficient transfers by eliminating crushing of agarose

gels. PCR* fragments, plasmid, and vector DNA ranging in size from several hundred bases

to 15 kilobases can be transferred in as little as 10 minutes. Set-up time is reduced to just

minutes because hydrolyzation of DNA fragments is not required prior to transfer. The blot-

ting kit can also transfer RNA to 3.5 kb in 30-35 minutes, although transfer of 28s RNA is

equally efficient as determined by EtBr staining.

Read the Trans-Blot SD transfer cell instruction manual thoroughly before per-

forming semi-dry electrophoretic DNA or RNA transfers.

1.1 Specifications

Construction:

Gel support frame Molded polycarbonate

Overall frame size: 16 x 21 cm

Maximum gel size: 15 x 20 cm

Cleaning: Use mild soap and warm water to clean the gel support frame.

For cleaning the Trans-Blot SD semi-dry electrophoretic transfer

cell, refer to the semi-dry cell instruction manual.

Chemical compatibility: The gel support frame is not compatible with TCA (trichloroacetic

acid), chlorinated hydrocarbons (e.g. chloroform), aromatic

hydrocarbons (e.g. toluene, benzene), or acetone. Use of such

organic solvents voids all warranties.

NOTE: The kit is intended for gels with dimensions of up to 15 x 20 cm. To blot a unique

size gel, adjustments must be made in transfer membrane and blot paper dimensions. For

questions regarding the proper assembly and operation of the Trans-Blot SD semi-dry

electrophoretic transfer cell, refer to the instruction manual provided with the instrument.

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Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual Page 4