Bio-rad Personal Molecular Imager™ (PMI™) System User Manual

Hardware Instruction Manualfor Catalog Number170-9400For Technical Service, Call Your Local Bio-Rad Office or, in the US, Call 1-800-4BIORAD (1-800-42

Fig. 2.3. External Laser Module(5) Sample TrayThe glass sample tray that is included with the PMI system, is used as a scanning platformfor all K-typ

2.3 Theory of Operation2.3.1 Storage Phosphor Detection MechanismWhen a radioactive emission strikes the BaFBr:Eu screen, phosphor oxidation occurs

8Fig. 2.5. Two and three-dimensional representations of a digitized image.Note: Storage phosphor screens are reusable after erasure.2.4 Overview of

Fig.2.4.1. Phosphor Imaging work flow.Section 3System Installation3.1 Operating Requirements3.1.1 System LocationThe PMI scanner and all peripheral

Screen EraserThe screen eraser has no minimum clearance requirement and may even be wall mounted,using the mounting holes located in the unit's b

Please refer to your software manual for detailed host computer system and softwarerequirements. If the computer is not purchased from Bio-Rad, system

scan head during transport and must be removed before power is supplied to the scanner. Ifthe screw is not removed the scanner may be damaged. To remo

Retain all packaging for screen storage. 3.2.3 Electrical and Communication ConnectionsPowerInsert the power cord into the power entry module on the

14The PMI scanner should be on for at least 40 seconds before the host computer is turnedon to allow for complete initialization of the laser scanning

15When screens are not in use, they should be placed in the original shipping box providedand placed in a dry and dark environment.Caution: The phosph

WelcomeDear Customer,On behalf of Bio-Rad Laboratories, we would like to thank you for investing in the PersonalMolecular Imager (PMI) system and we a

Dry Radioactive SamplesDry thin samples such as nitrocellulose membranes, dried gels, or TLC sheets can beexposed directly to the imaging screen in th

17Fig. 4.5. Protective sheet placement steps.Wet Radioactive SamplesPrecautions must be taken to prevent wet samples from contaminating the imaging s

184.2.4 How to Use the Exposure CassetteExposure Cassette-KFig. 4.6. Steps in use of the exposure cassette-K.• To open the cassette, push the two rel

19Note: When scanning a small sized screen it is recommended that the appropriate locationtemplate be used. This template will correctly align the sm

Scanning the Samples1. Start Quantity One software on computer.2. Open the scan window. Under the File menu, choose Select and use the layered menuto

5.2 Care for PMI Accessories5.2.1 General Care of Imaging ScreensUtmost care should be taken to ensure that the protective plastic covering over the

Cleaning Protocol for the Imaging Screen-K/Tritium Caution: The phosphor matrix of the tritium screen has no protective barrier and shouldNOT be clea

Step 6.Step 7a Step 7bFig. 5. Screen Eraser bulb replacement steps.Caution: Use only 15 W bulbs. Higher wattage bulbs can damage the imaging screens

Section 6Troubleshooting6.1 Factors Affecting Image QualityFluorescent samplesResolutionClose contact of the sample with the active surface of the im

25Appendix 1Personal Molecular Imager SpecificationsSystem Technical Specifications SpecificationLinear dynamic range 1:65535Pixel resolution 50, 100,

Table of ContentsSection 1 General Information ...11.1 About this Manual...

Appendix 2Personal Molecular Imager Warranty InformationThe Personal Molecular Imager system and all peripheral items are warranted for a periodof on

Appendix 3Ordering InformationAccessoriesCatalog # DescriptionSample Handling170-7811 Sample Tray170-7812 Multi-Sample Tray I, for small aluminum-moun

Life ScienceGroup00-000 0000 Sig 1204Bulletin 0000 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA (800) 4BIORAD Austr

Appendix 1 System Specifications ...25Appendix 2 Warranty Information ...

Section 1General Information1.1 About this ManualThis manual provides instructions for installing, operating and maintaining the PersonalMolecular Im

1.2.2 General WarningsWarning: There are hazardous voltages inside the PMI scanner. Do not attempt to defeatthe door interlock or remove the instrum

Warning: Do not remove the cover for any reason or defeat the interlock. Attempting tooperate the unit with the cover removed may damage the instrumen

4The PMI system (170-9400) is shipped together with following components as shown onthe Fig. 2.1.• PMI laser scanner• Quantity One software for image

5Table 2.1. Imaging Screen Specifications and RecommendedApplications(2) Sample Exposure CassettesThe sample exposure cassettes ensures that close co