Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual download pdf (Page 8)

Catalog

Number Product Description

Total Protein Detection Kits

170-6512 Biotin-Blot Protein Detection Kit

170-6517 Enhanced Colloidal Gold Total Protein Detection Kit

Blotting Standards

161-0372 Precision Prestained Standards, 10–250 kD, 500 µl

161-0380 Precision Streptactin-HRP conjugate

161-0381 Precision Streptactin-AP conjugate

161-0305 Prestained SDS-PAGE Standards, Low range

161-0309 Prestained SDS-PAGE Standards, High range

161-0307 Biotinylated SDS-PAGE Standards Kit, Low range, HRP

161-0308 Biotinylated SDS-PAGE Standards Kit, Low range, AP

161-0312 Biotinylated SDS-PAGE Standards Kit, High range, HRP

161-0313 Biotinylated SDS-PAGE Standards Kit, High range, AP

161-0306 Biotinylated SDS-PAGE Standards, Low range, 250 µl

161-0311 Biotinylated SDS-PAGE Standards, High range, 250 µl

Premixed Electrophoresis and Blotting Buffers

161-0732 10X Tris/Glycine/SDS, 1 L

161-0755 10X Tris/Glycine/SDS, 6 x 1 L

161-0734 10X Tris/Glycine, 1 L

161-0757 10X Tris/Glycine, 6 x 1 L

161-0733 10X Tris/Boric Acid/EDTA, 1 L

161-0756 10X Tris/Boric Acid/EDTA, 6 x 1 L

161-0741 10X TBE Extended Range, 1 L

161-0758 10X TBE Extended Range, 6 x 1 L

Section 3

Safety Instructions

Read the entire manual before beginning electrophoretic transfers.

Electrophoretic transfer of proteins and nucleic acids is dependent on many factors.

Observe the following guidelines to avoid mishaps that may result in serious damage to the

instrument or injury to the operator.

1. Do not reverse polarity on this instrument. This will result in corrosion and rusting of

the stainless steel cathode. If this should occur, the stainless steel should be cleaned with

a mild abrasive cleaner to remove the rust.

2. Do not exceed 25 V with this instrument. This could damage the electrodes.

3. Do not adjust the pH of transfer buffers unless specifically indicated. Follow instruc-

tions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in

increased buffer conductivity. This is manifested by a higher than expected initial current

output as shown by the power supply's current meter. Monitor buffer resistance with the

Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.

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Bio-rad Trans-Blot® SD Semi-Dry Transfer Cell User Manual Page 8