Bio-rad ProteinChip Serum Fractionation Kits User Manual

Biomek is a trademark of Beckman Coulter, Inc. HyperD is a trademark of PallCorporation. MicroMix is a trademark of Diagnostic Products Corporation.Th

© 2006 Bio-Rad Laboratories, Inc.© 2006 Bio-Rad Laboratories, Inc.IntroductionThe ProteinChip serum fractionation kit is designed to facilitate theana

© 2006 Bio-Rad Laboratories, Inc.© 2006 Bio-Rad Laboratories, Inc.nMicroplate sealing strips, 10 nInstruction manualNote: The volume of supplied reage

© 2006 Bio-Rad Laboratories, Inc.3. With an 8-channel pipet, add 200 µl of rehydration buffer toeach well.4. Carefully seal the plates with microplate

© 2006 Bio-Rad Laboratories, Inc.Step 6: Fraction CollectionNote: To avoid cross-contamination between wells, apply adhesive sealing film on themicrop

© 2006 Bio-Rad Laboratories, Inc. © 2006 Bio-Rad Laboratories, Inc.Fraction 41. Add 100 µl of wash buffer 4 to each well of the filtration plate.2. Mi

© 2006 Bio-Rad Laboratories, Inc. © 2006 Bio-Rad Laboratories, Inc.50,000 100,000 150,000 200,00001.000.51.001.001.000.20.40.601.02.03.0Fraction 6Frac

3. Enter the Gain Factor.The Gain Factor is the scaling of the amplitude of shake. It isadjusted by changing the multiplication factor used by thefirm