BIO RAD S3™ Cell Sorter manuals

Bio rad S3™ Cell Sorter User Manual (36 pages)

Brand: Bio-rad | Category: Measuring instruments | Size: 4.08 MB |

English

Table of contents

Biosafety System Class I

1

Legal Notices

3

Bio-Rad Technical Support

3

Safety Warning Labels

5

Instrument Warning Labels

5

Regulatory Compliance

6

Biohazards

6

Table of Contents

9

Introduction

11

System Components

12

Hardware Overview

13

Specifications

14

Operation

15

Software Control

16

Instruction Manual

17

Using the Access Doors

18

Fluidics Access Door

19

Vinyl Side Panel Operation

20

Maintenance

21

Filter Replacement

22

Service and Certification

23

Certification Procedure

24

Certiﬁcation

25

References

25

16

26

Contaminant Testing

27

18

28

Reference

29

Contaminant Testing Record

30

Troubleshooting

31

22

32

Ordering Information

33

Life Science

36

Bio-Rad

36

Laboratories, Inc

36

Bio rad S3™ Cell Sorter User Manual (132 pages)

Brand: Bio-rad | Category: Measuring instruments | Size: 28.83 MB |

English

Table of contents

Cell Sorters

1

Legal Notices

3

Bio-Rad Technical Support

3

Safety Warning Labels

5

Instrument Warning Labels

5

Regulatory Compliance

6

Biohazards

6

Table of Contents

9

Introduction

11

1.1 System Components

12

1.2 Installation Requirements

12

Hardware Description

13

2.1.1 Instrument Back Panel

14

2.1.2 Aerosol Evacuation Port

14

2.2 Fluidics System

15

2.2.2 Loading Stage

16

Instruction Manual

17

2.2.3 Nozzle

18

2.2.4 Sort Collection Chamber

18

2.3 Optics

19

2.4 Electronics

21

Software

23

3.1 Main Software Window

24

3.1.1 Title Bar Buttons

25

3.1.2 File Menu

25

3.1.3 Home Tab Toolbar

26

3.2 Control Panel

30

3.3 Administrator Tab Toolbar

32

3.3.1 Editing QC Criteria

33

3.3.2 Edit Droplets

34

3.3.3 Edit Streams

34

Instruction Manual

35

Test Pattern highlighted

35

3.3.4 User Reports

36

3.3.5 Managing Users

37

3.3.6 Decontaminate

38

3.3.8 Global Preferences

39

Boost Adjustment

40

Force Cleaning

40

Default Plot Statistics

40

Plot Defaults

40

Parameter Names

41

Default Save Location

41

Emergency Contact Information

41

Getting Started

43

4.2 Logging In

44

4.3 Daily Startup

45

4.4 Quality Control

46

4.4.1 Droplet Setup

47

4.4.2 Side Streams

48

4.4.3 Alignment

49

4.4.5 Drop Delay Maintenance

50

4.4.6 Recalculate Drop Delay

51

4.4.7 QC Criteria

51

4.4.8 QC Report

51

4.5 Protocols and Workspace

52

4.5.3 Saving Protocols

53

4.5.5 Opening FCS Files

54

4.5.6 Saving FCS Files

55

4.5.10 Changing Parameters

56

4.5.11 Viewing Statistics

57

4.5.13 Creating Regions

58

4.5.14 Creating Gates

59

Acquisition

61

5.1.1 Target Event Rate

62

5.1.2 Event Limit

63

5.1.3 Cycle Mode

63

5.1.4 Trigger

63

5.1.7 Auto Save

64

5.1.5 Threshold

64

5.1.6 PMT Voltages

64

5.2 Compensation

65

Instruction Manual

67

Fig. 61. Compensation matrix

67

5.2.2 Manual Compensation

69

5.3.1 Hot Swap

70

Instruction Manual

71

5.4 Optical Filters

72

5.4.2 Optical Filter Blocks

73

6.1 Sort Setup

75

Fig. 70. Sort Logic window

76

6.1.1 Sort Collection

77

6.1.2 Volume Tracker

77

6.1.3 Setting Regions to Sort

78

6.2 Sort Modes

79

6.3 Sort Statistics

79

6.4 Sort Plots

81

Additional Software Features

83

7.4 Clean System

84

7.5 Instrument Status Box

85

7.6 Status Bar

85

7.8 Quality Control Reports

86

7.7 Printing

86

7.9 User Reports

87

7.10 Biosafety System

88

Shutdown

89

Instruction Manual

91

8.1 Daily Shutdown

91

Automatic Startup

93

10.2 Dealing with Clogs

98

Maintenance

100

10.4 Swap Nozzle Tip Wizard

101

Instruction Manual

101

Instruction Manual

103

Instruction Manual

105

Cleaning the nozzle tip:

108

Sonication:

108

Syringe wash:

108

Instruction Manual

109

Instruction Manual

111

Instruction Manual

113

10.5 Optical Filter Cleaning

115

10.6 Disinfectants

115

10.7 Decontamination

115

Instruction Manual

119

Troubleshooting

121

References

125

and S3e

127

Cell Sorter

127

Specifications

127

Ordering Information

129

Life Science

132

Bio-Rad

132

Laboratories, Inc

132

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