BIO RAD ChromLab™ Software manuals

Bio rad ChromLab™ Software User Manual (306 pages)

Brand: Bio-rad | Category: Measuring instruments | Size: 2.91 MB |

English

Table of contents

Chromatography Systems

1

Software

1

Instrument Guide

1

NGC™ Chromatography

3

Systems and

3

Instrument Guide

7

Instrument Guide

9

Table of Contents

10

Instrument Guide

11

1 Introduction

13

(on the NGC Quest

14

Plus system)

14

Main Features

15

Site Requirements

16

Environment

17

Bench Space

17

Safety Requirements

18

Finding Out More

19

1

20

2 The NGC Instrument

21

2

22

Instrument Guide

23

Front View

24

Instrument Guide

25

Back View

26

Right Side View

27

Left Side View

28

System Pumps

31

Detailed Attributes

32

System Pump LEDs

32

How the System Pumps Function

32

Pump Priming Port

33

Piston Wash System

34

Sample Pump

35

Sample Pump LEDs

36

How the Sample Pump Works

36

Instrument Guide

37

Sample Inject Valve

39

Port Function

40

Sample Inject Valve LEDs

41

Valve Position Explanation

42

Buffer Blending Valve LEDs

46

Buffer Blending Mode

47

Gradient Mode

47

Inlet Valves

48

Inlet Valve

49

Ports on the Inlet Valves

50

Inlet Valve LEDs

51

How the Inlet Valves Work

52

Outlet Valve

53

Ports on the Outlet Valve

54

Outlet Valve LEDs

55

How the Outlet Valve Works

56

Column Switching Valve LEDs

60

Instrument Guide

61

Instrument Guide

63

Detectors

66

Instrument Guide

67

Single-Wavelength UV Detector

68

Instrument Guide

69

Conductivity Detector

71

How They Work

72

The Backpressure Regulator

73

Ports on the pH Detector

75

How the pH Detector Works

76

Instrument Guide

77

Pressure Rating

78

Mounted

78

Sample Injection Loops

79

Fittings

80

LEDs and LED Screens

81

Air Sensors

82

Instrument Guide

83

Air Sensor LEDs

84

Pressure Sensors

85

Temperature Sensor

85

NGC Touch Screen

86

Buffer Tray

87

Product Configurations

88

Discover series

89

3 Preparing the Instrument

91

Module Review

92

Instrument Guide

93

NGC Systems Accessories Kit

94

NGC Scout Accessories Kit

95

NGC Discover Accessories Kit

95

Buffer Bottles

96

Waste Bottles

96

Instrument Guide

97

Preparing the Detectors

99

Changing the Mixer Barrel

100

Barrel Size Flow Rate, ml/min

101

F10 Pump F100 Pump

101

(air sensor to pump inlet)

103

To activate the air sensors

105

Instrument Guide

107

Instrument Guide

109

Instrument Guide

111

Instrument Guide

113

Trigger wire

115

Positive

115

Starting the NGC Instrument

116

Instrument Guide

117

To verify the plumbing path

119

To prime the system pumps

121

To purge the system pumps

122

Instrument Guide

123

Priming the NGC Scout System

124

Instrument Guide

125

Purging the NGC Scout System

126

Instrument Guide

127

Priming NGC Discover Systems

128

Instrument Guide

129

Instrument Guide

131

To prime the sample pump

132

Instrument Guide

133

Instrument Guide

135

Instrument Guide

137

To purge the sample pump

138

Instrument Guide

139

Instrument Guide

141

Preparing the Columns

142

Instrument Guide

143

 UV monitor

144

Washing the Columns

145

Calibrating pH

147

To zero the pressure

148

Instrument Guide

149

A Maintaining the Instrument

151

Storing the NGC Instrument

153

Interval Component Action

154

As Needed

156

Component Solution to use

157

System and Sample Pumps

158

Pump Head Assembly

159

Piston Wash Assembly

160

Disassembling the Pumps

161

Instrument Guide

163

To clean the pump heads

164

Instrument Guide

165

Instrument Guide

167

To replace the pump head seal

169

Removing the Wash Housing

171

Removing the Piston

172

Instrument Guide

173

Instrument Guide

175

Reassembling the Pumps

177

Installing the Wash Housing

178

Instrument Guide

179

Installing the Pump Heads

180

Instrument Guide

181

Instrument Guide

183

Instrument Guide

185

Conditioning New Seals

187

Instrument Guide

189

Disassembling the Mixer

190

Instrument Guide

191

To change the O-rings

193

To clean the mixer body

195

Reassembling the Mixer

196

Instrument Guide

197

The Detectors

198

Instrument Guide

199

Instrument Guide

201

Instrument Guide

203

Instrument Guide

205

Instrument Guide

207

Back of LED Board

209

Front of LED Board

209

To reset lamp time details

210

Instrument Guide

211

6. Remove the lamp:

213

Instrument Guide

215

Instrument Guide

217

Storing the pH Electrode

219

Cleaning the pH Electrode

219

Replacing the pH Probe

220

Instrument Guide

221

Other Components

222

Instrument Guide

223

Fourth expansion tier

224

Previously attached

224

For example:

225

Instrument Guide

227

Task: Refer to the section:

228

Instruments

229

Bottom of Module

231

Top of Module

231

Instrument Guide

233

Instrument Guide

235

Cutting Replacement Tubing

236

Instrument Guide

237

Instrument Guide

239

Instrument Guide

241

Instrument Guide

243

Instrument Guide

245

B Troubleshooting the

247

Instrument

247

Instrument Guide

249

 Perform CIP to clean the

250

 Replace the pump seals

250

 Replace the check valves

250

Instrument Guide

251

 Replace check valves

252

Instrument Guide

253

UV or UV/Vis Detector

255

 Use the lamp usage values

256

 See Replacing the Single

256

 See Replacing the Multi

256

Instrument Guide

257

 Recalibrate both system

258

Instrument Guide

259

Conductivity Monitor

260

Instrument Guide

261

Buffer Blending Valve

262

Column Switching Valve

262

External Devices

263

C Plumbing the System

265

Plumbing NGC Systems

266

Plumbing All NGC Systems

267

System Pumps Wash Flow

268

Sample Pump Wash Flow

268

Instrument Guide

269

To plumb the sample pump

270

Instrument Guide

271

C

272

Plumbing the NGC Quest System

273

To plumb the system pumps

275

Plumbing the NGC Scout System

276

Instrument Guide

277

To plumb the pH valve

278

Instrument Guide

279

Instrument Guide

281

Tubing color Port Solution

282

Instrument Guide

285

Plumbing Sample Inlet Valves

287

Plumbing Outlet Valves

288

Instrument Guide

289

D Connecting the C-96

291

Instrument Guide

293

To prepare the tubing

294

Autosampler

295

Sample Inject

295

Instrument Guide

297

E Regulatory Information

299

5 ml F10 2,650 psi

300

5 ml F100 1,400 psi

300

DynaLoop™ 25 ml F100 700 psi

300

DynaLoop 90 ml F100 270 psi

300

FCC Warning and Notes

301

F NGC Chromatography

303

Systems Catalog Numbers

303

Life Science

306

Bio-Rad

306

Laboratories, Inc

306

Bio rad ChromLab™ Software User Manual (300 pages)

Brand: Bio-rad | Category: Measuring instruments | Size: 4.20 MB |

English

Table of contents

Chromatography Systems

1

Software

1

User Guide

1

NGC™ Chromatography

3

Systems and

3

User Guide

7

User Guide

9

Table of Contents

10

User Guide

11

1 Introduction

13

Main NGC Features

14

User Guide

15

NGC Chromatography Systems

16

User Guide

17

Finding Out More

18

2 The Workspace

19

The Home Window

20

User Guide

21

User Guide

23

The System Control Window

25

The Method Editor Window

26

The Evaluation Window

27

2

28

Touch Screen Menu Commands

29

Touch Screen Toolbar Commands

30

3 System Control

31

3

32

Manual Menu Commands

34

Tools Menu Commands

34

Context Menu Commands

35

Chromatogram View

37

Table 1. Trace Definitions

39

Trace Module Explanation

40

Showing or Hiding Traces

41

Changing Trace Color

42

Changing the Y-Scale Values

43

Autoscaling the UV Trace

43

To add annotations

45

To edit an annotation

45

To delete an annotation

45

User Guide

47

Fluidic Scheme Pane

48

User Guide

49

Sample Inject Valve

51

Sample Pump

52

Column Switching Valve

52

UV/Conductivity Monitors

52

Signal Import Module

52

Fluidic Scheme Configurations

53

User Guide

55

User Guide

57

Working with Fluidic Schemes

59

To change module settings

61

Fluidic Scheme Mapping

62

Color Label valve

63

To unmap valves

64

To cancel unmapping valves

64

To map valves

65

Calibrations

66

User Guide

67

Calibrating Pressure Settings

68

User Guide

69

To verify the plumbing path

70

System Settings

71

To set the delay volume

72

Orange 0.02" (0.5 mm)

73

Green 0.03" (0.75 mm)

73

Clear 0.062" (1.6 mm)

73

Control Flow Tab

74

To control the flow rate

75

Remote Access Tab

76

Trace Settings Tab

76

User Guide

77

Device Input Tab

78

User Guide

79

Device Output Tab

80

To disable the connection

81

Air Sensors Tab

82

To deactivate an air sensor

83

Email Notifications Tab

84

User Guide

85

System Name Tab

87

System Information

88

Detector Tab

89

Multi-Wavelength Detector

90

Preferences

91

User Guide

93

4 Performing a Manual Run

95

User Guide

97

User Guide

99

Preparing the System

101

User Guide

101

User Guide

103

Cleaning the System

104

Running an Experiment

105

User Guide

107

User Guide

109

To run an experiment step

110

Changing Module Settings

111

Stopping a Manual Run

111

Clearing Run Data

111

Saving a Manual Run

111

Viewing Run Data

113

5 Method Editor

115

Before You Begin

116

Opening a Method

117

5

118

File Menu Commands

119

Edit Menu Commands

120

View Menu Commands

121

Help Menu Commands

121

Toolbar Commands

121

Phase Library Pane

122

Adding a Phase to a Method

123

Method Outline Pane

124

Phase Parameters Pane

124

Method Steps Pane

125

To view step details

126

Method Settings Parameters

127

Column Selection

128

User Guide

129

To add user-defined columns

131

Detector Settings

133

Unit Selection

133

Fraction Collection

134

Buffer Selection

135

Pump Inlets

136

Pump + Inlet Valves

136

Pump + Buffer Blending

136

To select a buffer

137

Phase Controls and Parameters

138

User Guide

139

Standard Phases

140

User Guide

141

User Guide

143

Column Wash Parameters

145

Elution Parameters

146

Special Considerations

147

NGC Scout Systems

148

User Guide

149

User Guide

151

To clean the pH valves

152

User Guide

153

User Guide

155

System Preparation Parameters

156

Create New Phase Parameters

157

Step Library Pane

158

Scout Parameters Pane

159

6 Creating a Method

161

Standard Method Templates

162

User Guide

163

6

164

User Guide

165

User Guide

167

To create a method

168

To change the fluidic scheme

168

To specify general settings

169

User Guide

171

Adding Phases

172

Editing Methods and Phases

172

Renaming Phases

173

Rearranging Phases

173

Deleting Phases

174

User Guide

175

To run a method

177

To add runs to the run queue

177

Saving a Method

179

Renaming a Method

180

Deleting a Method

181

The Scouting Wizard

183

User Guide

185

User Guide

187

User Guide

189

Creating a Scouting Method

191

User Guide

193

Running a Scouting Method

194

To run a scout method

195

7 Evaluating Results

197

Managing Analysis Projects

198

To open a saved analysis

199

7

200

Analysis Menu Commands

203

Tab Toolbar Commands

204

Customizing the Chromatogram

205

Selecting the Active Trace

206

Showing or Hiding a Trace

207

Changing the Axes

207

Changing Trace Colors

208

Zooming In and Out

209

Chromatogram

210

To resize the selected region

211

Annotating the Chromatogram

212

Copying the Chromatogram

213

Changing Table Grouping

214

Sorting Table Columns

215

Copying a Table

216

User Guide

217

Managing Runs

218

To close a tab

219

To close all tabs

219

Comparing Traces

220

Stacked View

221

Overlay View

223

Hiding All Traces in a Group

224

Showing All Traces in a Group

224

To offset a trace

225

Managing Analyses

226

Copying an Analysis

227

Renaming an Analysis

227

Exporting Run Data

228

Peak Integration

229

Baseline Parameters

230

Peak Parameters

231

Peak Filtering

231

Default Parameters

231

Starting Peak Integration

232

User Guide

233

Changing Peak View Options

234

User Guide

235

Showing or Hiding Columns

237

Copying the Peaks Table

238

User Guide

239

Adjusting Peaks Manually

240

To add a peak

241

Saving Peak Integration Data

242

Evaluating Fractions

243

Rack Display

244

Outlet Valve Display

244

Fractions Table

245

User Guide

247

Viewing Fraction Details

248

Pooling Fractions

248

To create a fraction pool

249

To clear a pool

249

Viewing Heat Map Details

251

To hide the rack display

252

Column Performance Analysis

253

Calculate

254

Analyzing Column Performance

255

 Click Delete

256

Column Performance Table

258

User Guide

259

8 Importing and Exporting

261

To import a method or run

262

Importing Unicorn Data Files

263

User Guide

265

Exporting Data

266

Exporting Data as an NGC File

267

To export only the method

268

To export only the run data

268

User Guide

269

To export multiple runs

270

User Guide

271

Exporting Diagnostic Logs

273

 Change the date range

274

User Guide

275

9 Reports

277

Producing a Report

278

Method Reports

279

Run Reports

279

Printing a Report

280

Saving a Report

281

9

282

A Database Management

283

Back Up the NGC Database

284

Restore the NGC Database

285

A

286

B Multicolumn Purifications

287

 Method base unit

288

 Sample volume

288

 Step duration and length

288

User Guide

289

Template Name Description

291

Plumbing the NGC System

293

Purification Templates

294

User Guide

295

Priming the NGC System

296

User Guide

297

Life Science

300

Bio-Rad

300

Laboratories, Inc

300

Bio rad ChromLab™ Software User Manual (178 pages)

Brand: Bio-rad | Category: Measuring instruments | Size: 2.01 MB |

English

Table of contents

Chromatography Systems

1

Software

1

Installation Guide

1

NGC™ Chromatography

3

Systems and

3

ChromLab™ Software

3

Table of Contents

5

Installation Guide

7

1 Introduction

9

Main Features

10

Safety Information

11

Regulatory Information

12

FCC Warning and Notes

13

Finding Out More

14

2 Preparing Your Site

15

Item Specification

16

Room Requirements

17

Environment

18

Power Requirements

19

ChromLab System Requirements

20

Installation Guide

21

To turn off automatic updates

22

To change the font size

22

3 Setting Up NGC

23

Chromatography Systems

23

NGC Systems Accessories Kit

24

NGC Scout Accessories Kit

25

NGC Discover Accessories Kit

25

Attaching the Touch Screen

26

Installation Guide

27

Installation Guide

29

Installation Guide

31

Installation Guide

33

Installation Guide

35

Installation Guide

37

Stand kit (#788-5040)

38

Installation Guide

39

Installation Guide

41

Installation Guide

43

To install the pH probe

44

Installation Guide

45

Installation Guide

47

Upper expansion tier

48

Previously attached

48

Installation Guide

49

Installation Guide

51

Task: Refer to the section:

52

Inserting Additional Modules

53

Installation Guide

55

Installation Guide

57

 Connecting the Air Sensors

58

Installation Guide

59

Installation Guide

61

 UV monitor

62

Installation Guide

63

Connecting the Air Sensors

64

(air sensor to pump inlet)

65

To activate the air sensors

67

Installation Guide

69

Installation Guide

71

Installation Guide

73

Installation Guide

75

Trigger wire

77

Positive

77

Installation Guide

79

Installation Guide

81

4 Installing ChromLab

83

Software

83

Installing ChromLab Software

84

The wizard installs ChromLab

85

Software and the NGC Systems

86

Installation Guide

87

Setting the System Name

88

Installation Guide

89

On the ChromLab Computer

90

ChromLab computer

91

On the NGC Instrument

92

Installation Guide

93

Setting Delay Volume

95

Installation Guide

97

5 Plumbing and Priming the

99

Buffer Bottles

100

Waste Bottles

100

Plumbing NGC Systems

101

Plumbing All NGC Systems

102

System Pumps Wash Lines

103

Sample Pump Wash Line

103

To plumb the sample pump

105

Installation Guide

107

Plumbing the NGC Quest System

108

Installation Guide

109

To plumb the system pumps

110

Plumbing the NGC Scout System

111

To plumb the pH valve

113

Installation Guide

115

Tubing color Port Solution

117

Installation Guide

121

Plumbing Sample Inlet Valves

122

Plumbing Outlet Valves

123

Installation Guide

125

To prime the system pumps

126

To purge the system pumps

127

Priming the NGC Scout System

129

Purging the NGC Scout System

131

Priming NGC Discover Systems

133

Installation Guide

135

To prime the sample pump

137

Installation Guide

139

Installation Guide

141

To purge the sample pump

143

Installation Guide

145

Calibrating the Instrument

146

To zero the pressure

147

A Upgrading ChromLab

149

Updating NGC Systems

150

Installation Guide

151

B Troubleshooting ChromLab

153

Possible Cause Solution

154

Installation Guide

157

Installation Guide

159

Installation Guide

161

Installation Guide

163

NGC ChromLab Application

164

Firmware Update Failure

165

C Connecting the C-96

167

Installation Guide

169

To prepare the tubing

170

Autosampler

171

Sample Inject

171

Installation Guide

173

D Firewall Configuration

175

Life Science

178

Bio-Rad

178

Laboratories, Inc

178

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