Bio-rad ChromLab™ Software User Manual download pdf (Page 137)

Priming and Purging the System

Installation Guide | 135

9. Repeat steps 4–8 for the remaining buffer inlet ports.

10. To prime buffer inlet valve B, select buffer B1 and perform steps 3–9 on system

pump B.

To prime the sample pump

1. Immerse the inlet tubing for the sample pump in the appropriate container.

2. Insert a 30 ml syringe into the priming port.

3. Turn the priming port valve counter

-clockwise one full turn to open the seal.

4. Slowly withdraw the

plunger to withdraw liquid from the port

until

air no

longer enters the syringe (approximately 10 ml)

and all tubing is filled with

liquid.

5. Turn the priming port valve clockwise to close the port.

6. Remove the syringe from the priming port and discard the liquid into waste.

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Bio-rad ChromLab™ Software User Manual Page 137