Bio-rad ChromLab™ Software User Manual Page 69
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Detectors
Instrument Guide | 6 7
Monitors UV absorbance one wavelength at a time
255 nm
280 nm (default)
Tip: Use ChromLab to change the absorbance wavelength values. For
more information, see the NGC Chromatography Systems and ChromLab
Software User Guide.
UV absorbance range: 0–3,000 mAU
UV linearity: ±5%
Three interchangeable flow cells with path lengths of 2 mm, 5 mm (default),
and 10 mm; see Table 4 on page 69 for specific information
Note: Use the preparative 2 mm flow cell to measure high concentrations
of protein. The path lengths of the analytical 5 mm and 10 mm flow cells
are longer and are appropriate for measuring lower concentrations of
protein.
For information about changing the flow cells, see Replacing the UV Flow Cell
on page 196.
- Chromatography Systems 1
- Software 1
- Instrument Guide 1
- NGC™ Chromatography 3
- Systems and 3
- Instrument Guide 7
- Instrument Guide 9
- Table of Contents 10
- Instrument Guide 11
- 1 Introduction 13
- (on the NGC Quest 14
- Plus system) 14
- Main Features 15
- Site Requirements 16
- Environment 17
- Bench Space 17
- Safety Requirements 18
- Finding Out More 19
- 1 20
- 2 The NGC Instrument 21
- 2 22
- Instrument Guide 23
- Front View 24
- Instrument Guide 25
- Back View 26
- Right Side View 27
- Left Side View 28
- System Pumps 31
- Detailed Attributes 32
- System Pump LEDs 32
- How the System Pumps Function 32
- Pump Priming Port 33
- Piston Wash System 34
- Sample Pump 35
- Sample Pump LEDs 36
- How the Sample Pump Works 36
- Instrument Guide 37
- Sample Inject Valve 39
- Port Function 40
- Sample Inject Valve LEDs 41
- Valve Position Explanation 42
- Buffer Blending Valve LEDs 46
- Buffer Blending Mode 47
- Gradient Mode 47
- Inlet Valves 48
- Inlet Valve 49
- Ports on the Inlet Valves 50
- Inlet Valve LEDs 51
- How the Inlet Valves Work 52
- Outlet Valve 53
- Ports on the Outlet Valve 54
- Outlet Valve LEDs 55
- How the Outlet Valve Works 56
- Column Switching Valve LEDs 60
- Instrument Guide 61
- Instrument Guide 63
- Detectors 66
- Instrument Guide 67
- Single-Wavelength UV Detector 68
- Instrument Guide 69
- Conductivity Detector 71
- How They Work 72
- The Backpressure Regulator 73
- Ports on the pH Detector 75
- How the pH Detector Works 76
- Instrument Guide 77
- Pressure Rating 78
- Mounted 78
- Sample Injection Loops 79
- Fittings 80
- LEDs and LED Screens 81
- Air Sensors 82
- Instrument Guide 83
- Air Sensor LEDs 84
- Pressure Sensors 85
- Temperature Sensor 85
- NGC Touch Screen 86
- Buffer Tray 87
- Product Configurations 88
- Discover series 89
- 3 Preparing the Instrument 91
- Module Review 92
- Instrument Guide 93
- NGC Systems Accessories Kit 94
- NGC Scout Accessories Kit 95
- NGC Discover Accessories Kit 95
- Buffer Bottles 96
- Waste Bottles 96
- Instrument Guide 97
- Preparing the Detectors 99
- Changing the Mixer Barrel 100
- Barrel Size Flow Rate, ml/min 101
- F10 Pump F100 Pump 101
- (air sensor to pump inlet) 103
- To activate the air sensors 105
- Instrument Guide 107
- Instrument Guide 109
- Instrument Guide 111
- Instrument Guide 113
- Trigger wire 115
- Positive 115
- Starting the NGC Instrument 116
- Instrument Guide 117
- To verify the plumbing path 119
- To prime the system pumps 121
- To purge the system pumps 122
- Instrument Guide 123
- Priming the NGC Scout System 124
- Instrument Guide 125
- Purging the NGC Scout System 126
- Instrument Guide 127
- Priming NGC Discover Systems 128
- Instrument Guide 129
- Instrument Guide 131
- To prime the sample pump 132
- Instrument Guide 133
- Instrument Guide 135
- Instrument Guide 137
- To purge the sample pump 138
- Instrument Guide 139
- Instrument Guide 141
- Preparing the Columns 142
- Instrument Guide 143
- UV monitor 144
- Washing the Columns 145
- Calibrating pH 147
- To zero the pressure 148
- Instrument Guide 149
- A Maintaining the Instrument 151
- Storing the NGC Instrument 153
- Interval Component Action 154
- As Needed 156
- Component Solution to use 157
- System and Sample Pumps 158
- Pump Head Assembly 159
- Piston Wash Assembly 160
- Disassembling the Pumps 161
- Instrument Guide 163
- To clean the pump heads 164
- Instrument Guide 165
- Instrument Guide 167
- To replace the pump head seal 169
- Removing the Wash Housing 171
- Removing the Piston 172
- Instrument Guide 173
- Instrument Guide 175
- Reassembling the Pumps 177
- Installing the Wash Housing 178
- Instrument Guide 179
- Installing the Pump Heads 180
- Instrument Guide 181
- Instrument Guide 183
- Instrument Guide 185
- Conditioning New Seals 187
- Instrument Guide 189
- Disassembling the Mixer 190
- Instrument Guide 191
- To change the O-rings 193
- To clean the mixer body 195
- Reassembling the Mixer 196
- Instrument Guide 197
- The Detectors 198
- Instrument Guide 199
- Instrument Guide 201
- Instrument Guide 203
- Instrument Guide 205
- Instrument Guide 207
- Back of LED Board 209
- Front of LED Board 209
- To reset lamp time details 210
- Instrument Guide 211
- 6. Remove the lamp: 213
- Instrument Guide 215
- Instrument Guide 217
- Storing the pH Electrode 219
- Cleaning the pH Electrode 219
- Replacing the pH Probe 220
- Instrument Guide 221
- Other Components 222
- Instrument Guide 223
- Fourth expansion tier 224
- Previously attached 224
- For example: 225
- Instrument Guide 227
- Task: Refer to the section: 228
- Instruments 229
- Bottom of Module 231
- Top of Module 231
- Instrument Guide 233
- Instrument Guide 235
- Cutting Replacement Tubing 236
- Instrument Guide 237
- Instrument Guide 239
- Instrument Guide 241
- Instrument Guide 243
- Instrument Guide 245
- B Troubleshooting the 247
- Instrument 247
- Instrument Guide 249
- Perform CIP to clean the 250
- Replace the pump seals 250
- Replace the check valves 250
- Instrument Guide 251
- Replace check valves 252
- Instrument Guide 253
- UV or UV/Vis Detector 255
- Use the lamp usage values 256
- See Replacing the Single 256
- See Replacing the Multi 256
- Instrument Guide 257
- Recalibrate both system 258
- Instrument Guide 259
- Conductivity Monitor 260
- Instrument Guide 261
- Buffer Blending Valve 262
- Column Switching Valve 262
- External Devices 263
- C Plumbing the System 265
- Plumbing NGC Systems 266
- Plumbing All NGC Systems 267
- System Pumps Wash Flow 268
- Sample Pump Wash Flow 268
- Instrument Guide 269
- To plumb the sample pump 270
- Instrument Guide 271
- C 272
- Plumbing the NGC Quest System 273
- To plumb the system pumps 275
- Plumbing the NGC Scout System 276
- Instrument Guide 277
- To plumb the pH valve 278
- Instrument Guide 279
- Instrument Guide 281
- Tubing color Port Solution 282
- Instrument Guide 285
- Plumbing Sample Inlet Valves 287
- Plumbing Outlet Valves 288
- Instrument Guide 289
- D Connecting the C-96 291
- Instrument Guide 293
- To prepare the tubing 294
- Autosampler 295
- Sample Inject 295
- Instrument Guide 297
- E Regulatory Information 299
- 5 ml F10 2,650 psi 300
- 5 ml F100 1,400 psi 300
- DynaLoop™ 25 ml F100 700 psi 300
- DynaLoop 90 ml F100 270 psi 300
- FCC Warning and Notes 301
- F NGC Chromatography 303
- Systems Catalog Numbers 303
- Life Science 306
- Bio-Rad 306
- Laboratories, Inc 306
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