BIO RAD 200/2.0 Instruction Manual

Trans-Blot®SDSemi-DryElectrophoreticTransfer CellInstructionManualCatalog Number170-3940For Technical Service Call Your Local Bio-Rad Office or in the

Low molecular weight macromolecules ( 10,000 daltons) may diffuse out of gels morereadily. One can allow adequate gel pre-equilibration by changing th

2. Place a pre-soaked sheet of extra thick filter paper onto the platinum anode. Roll a pipetor test tube over the surface of the filter paper (like a

7. Carefully place the cathode onto the stack. Press to engage the latches with the guideposts without disturbing the filter paper stack.8. Place the

4.3 Assembly of the Unit for Acidic TransfersIf an acidic transfer buffer is used, the transfer direction will be from the anode to thecathode.1. Rem

Note: Some pH electrodes will not perform a proper measurement for the pH of Trisbuffers. If the pH of the buffer is not correct, check the electrode

6.2 DNA Blotting(For acrylamide gels with DNA 250 bp to ~1 kb)Electrophoresis Run on a Polyacrylamide Gel1. Prepare the stock electrophoresis 5x TBE

Nitrocellulose membranes have been used extensively for protein binding and detec-tion.7,19-22They can easily be stained for total protein by a dye st

6. Methanol in the transfer buffer is restricting elution of proteins from the gel. Eliminationof methanol results in increased transfer efficiency, b

8.4 Poor Detection Sensitivity or No Reactivity1. Consult detection kit manual.2. Antigen binding is incomplete. See Troubleshooting Sections 8.1–8.3

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NoteTo insure the best performance from the Trans-Blot SD semi-dry electrophoretic transfercell, become fully acquainted with these operating instruct

Table of ContentsPageSection 1 Introduction... 11.1 Sp

Section 1IntroductionBlotting was first performed by Southern1in 1975 with the transfer of DNA from agarosegels to nitrocellulose membranes. Blotting

Section 2Equipment and Reagents2.1 Equipment and AccessoriesCatalogNumber Product Description170-3940 Trans-Blot SD Electrophoretic Transfer CellRepl

CatalogNumber Product DescriptionNitrocellulose Membrane (0.2 micron) Recommended uses162-0112 Roll, 33 cm x 3 m, 1162-0146 Sheets, 7 x 8.4, 10162-014

2.2 Related InstrumentsCatalogNumber Product DescriptionBlotting Equipment170-3910 Trans-Blot Electrophoretic Transfer Cell170-3946 Trans-Blot Electr

CatalogNumber Product DescriptionTotal Protein Detection Kits170-6512 Biotin-Blot Protein Detection Kit170-6517 Enhanced Colloidal Gold Total Protein

4. Lengthy transfer times are not recommended. Do not leave this instrument unattended.Joule heat can be generated rapidly during semi-dry blotting. T