Bio-rad Components for Older Model Spot Cutter User Manual

ProteomeWorks™Spot CutterFluorescent EnclosureInstruction ManualCatalog Numbers165-7045165-7044For technical servicecall your local Bio-Rad office ori

2.5 Spot Cutter InstallationIf you are installing the ProteomeWorks Spot Cutter, catalog number 165-7009, at the same timeas the Fluorescent Enclosure

1. Load the software CD into the CD drive and follow the onscreen instructions for installation.2. If you are using the "Basic Excision Tool&qu

2. The other version of lens has a silver-notched band and set-screw on the side of the lens. Thewhite dot below the silver notched band is the apertu

3.3 Checklist for Operation in UV Light Mode1. Toggle the white/UV light switch on the back of the ballast box on the spot cutter to theUV lights pos

This target is also useful for focussing the lens during installation, re-calibration, or forchecking the image acquisition in UV or white light modes

Section 4Fluorescent Operation in Basic Excision ToolThe PDQuest version 6.2.1 is very similar to version 6.2.0 in operating the spot cutter. There ar

4.3 Cutting Spots From Fluorescent Stained Gels in Basic Excision Tool1. Check that the light toggle switch is in UV lights position.2. Check that t

1. When UV-Fluorescent imaging has been used on the spot cutter, at the end of the cut runthe software will prompt with, "Confirm cuts?" Fi

7. Assess the image on the screen for the wells that need to be recut.Fig. 21. Microtiter plate with fluorescent spots in wells.Use the Zoom and tra

8. If any of the wells are empty and you want to re-cut those spots, click on “Specify re-cuts.”Then follow these on-screen instructions:Fig. 23. Me

Table of ContentsSSeeccttiioonn 11 IInnttrroodduuccttiioonn...11.1 General Descrip

4.5 Begin/Resume and Pause ButtonsThe Begin cut button has been modified in name to be more descriptive of the different opera-tions that may be used

7. You should be able to see "holes" in the spots in the image within the overlay target circles.However, there is a + or - 6% to 10% accura

5.2 Acquire Fluorescent Image in Integrated Excision Tool1. After the Cut List has been selected for spot cutting in the Excision Gel Selection scree

13. The image is automatically inverted by the software to show the gel as dark spots withlight background. If the original fluorescent image with lig

Answer "Yes" to bring up the following on-screen instructions for imaging the microtiter plate:Fig. 28. Image microtiter plate window in In

7. An image of the microtiter plate will open in the "Image Microtiter Plate" window.Fig. 29. Microtiter plate with fluorescent spots in w

8. If any of the wells are empty and you want to re-cut those spots, click on "Specify re-cuts." Then follow these on-screen instructions:F

16. Click on "Begin/Resume" after the selections are made. The “MTP Image“ window willclose. All the spots that have been marked for re-cut

7. You should be able to see "holes" in the spots in the image within the overlay target circles.However, there is a + or - 6% to 10% accura

Section 6Accessories6.1 Use of Gel Sheets and Gel PositioningThe Gel Sheets (reorder part number 165-7018) are designed to be used with individual gel

SSeeccttiioonn 66 AAcccceessssoorriieess...266.1 Use of Gel Sheets and Gel Positioni

7.3 Warranty InformationThis warranty statement may vary outside of the continental United States. Please contact yourlocal Bio-Rad office for the exa

System RecordsSerial Number Date of PurchasePurchase OrderNumberNotesService RecordDate Description of Problem Name Signature28

4006206 Rev BLife ScienceGroupWeb siteAlso in: AustraliaAustriaBelgium Ph. 09-385 55 11, Fx. 09-385 65 54Brazil Denmark FinlandFrancePh. 01 47 95 69

Section 1Introduction1.1 General DescriptionThis manual describes the installation, operation, and maintenance of the Fluorescent Enclosurefor the Pro

Fig. 1. Fluorescent Enclosure with spot cutter.1.5 SpecificationsNominal input voltage range 100 V to 240 V (50 Hz/60 Hz)Bandpass filter 620/60 nmUV

NNoottee::This Bio-Rad instrument is designed for laboratory use only and is certified to meet EN-61010 safety standards. Operation of this product is

2.2 Remove the DoorThe door is easily removed at the hinges for easier access to the interior while installing the lights,and for placing it over the

Fig. 5. Right side mounting brackets inside the Enclosure.3. Using the two large thumbscrews, attach the front light assembly bracket to the frontmo

62.4 Installing the Bandpass Filter1. The bandpass filter is shipped in the Filter Assembly box. It includes a filter holder, a620/60 nm bandpass fil