Bio-rad CHEF Genomic DNA Plug Kits User Manual

CHEF Genomic DNA Plug KitsInstruction ManualCatalog Numbers170-3591170-3592170-3593For Technical ServiceCall Your Local Bio-Rad Office orin the U.S. C

9.2 Agarose ConcentrationsVarious agarose plug percentages are reported in theliterature. The most common final concentration is 0.5%, butthe softness

A. Mammalian or Tissue Culture Cells:Because of the large size, tissue culture cells can becounted at 100x power. Count 10 of the large squares, five

LIT510 Rev ELife ScienceGroupWebsite www.bio-rad.com U.S. (800) 4BIORAD Australia 02 9914 2800 Austria (01) 877 89 01 Belgium 09-385 55 11 Canada

Table of ContentsSection 1 Introduction...11.1 Kit Components ...

Section 1IntroductionPulsed-Field Gel Electrophoresis (PFGE) allows theseparation of DNA ranging in size from a few kilobase pairsto 10 megabase pairs

170-3593 CHEF Yeast Genomic DNA Plug Kit, contains CellSuspension Buffer, 12 ml; >600 U/ml Proteinase K, 1.3 ml; Proteinase K Reaction Buffer, 30 m

7. Wash the plugs four times in 1x Wash Buffer, 1 hour eachat room temperature with gentle agitation (for each plug,use 1 ml of 1x Wash Buffer). Prepa

of Cell Suspension Buffer calculated above and equilibratethe cell suspension to 50 °C.7. Combine the calculated volume of 2% CleanCut agarosewith the

1. Inoculate a single colony into 50 to 100 ml YPD broth orappropriate media. Grow with agitation to an O.D.600of>1.0 at the appropriate temperatur

Note: Various cell lines have been incubated up to 4 daysin Proteinase K without detrimental effects to the qualityof DNA.11. Wash the plugs four time

Section 7Additional Reagents forPulsed Field ElectrophoresisCatalog Number Product Description162-0137 Pulsed Field Certified Agarose, 100 g162-0135 C