Bio-rad Experion Protein Analysis Kits User Manual

Experion™RNAStdSens Starter KitInstruction ManualCatalog Number700-7111For technical support call your local Bio-Rad office, or in the US call 1-800-4

Starter Kit Test 1 — RNA Analysis Basics1.1 Overview of Test 1Microfluidic chips use much smaller amounts and volumes of samples and reagents thantra

– RNA loading buffer (yellow cap)– RNA gel (green cap)2Notes: Experion RNA stain (blue cap) is light-sensitive and contains DMSO, which is highlyhygro

1.2.5 Prime the ChipBefore priming the chip, ensure that all samples, the RNA ladder, and other reagents areprepared and ready to be loaded. If a chi

8. Remove the chip from the priming station, turn it over, and inspect the microchannelsfor bubbles or evidence of incomplete priming. If the chip is

Fig. 1.3. Bubble formation during loading of Experion Pro260 chips. Left, example of bubblestrapped at the bottom of wells. The GS and G wells and sam

Fig. 1.4. Details of the New Run screen. The green dot in the lower right hand corner indicates thatcommunication between the electrophoresis station

Fig. 1.5. New Run dialog. The Experion system stops analysis when it reaches the number of samplesentered.7. Select the number of samples to be analyz

1.3 Data AnalysisEvaluate the performance of Test 1 and the analysis of the data by Experion software.1. Evaluate the virtual gel to ensure that all

3. Evaluate the separation of the RNA ladder (Figure 1.7). This is a critical step in dataanalysis because much of the automated data analysis perform

4. Examine the separation of the total RNA sample. Click on the sample name in the project tree or on a lane in the virtual gel. The electropherogram

About This ManualThis manual serves as an instruction manual for the Experion™RNA StdSens starter kit.General instructions for using the Experion syst

Fig. 1.9. Results table generated for well 2 in Test 1.6. To perform a qualitative evaluation of quantitation reproducibility, overlay the electropher

10. The data appear in the spreadsheet. Click on cell H1 (Peak Number) and select Data > Sort to sort the data by peak number.11. Insert 3 columns

Starter Kit Test 2 — Quantitation Across a Range ofConcentrations2.1 Overview of Test 2In this test, you analyze a dilution series of the total RNA s

2.3 Data AnalysisEvaluate the run and the analysis of the data by Experion software.1. Evaluate the virtual gel to ensure that all lanes (samples) ar

5. Evaluate the data analysis performed by Experion software. Click on the Results taband view the RNA concentrations given for each sample in the Res

Fig. 2.4. Excel spreadsheet showing Test 2 data sorted by peak number.8. Insert a column between columns H and I, and label it Exp Conc(ExpectedConcen

11. Plot the results as a function of sample concentration (Figure 2.7). The overall trendshould be linear.Fig. 2.7. Plot of concentration calculated

Appendix A — How the Experion™System Works The Experion electrophoresis system performs electrophoresis of samples within a microfluidicchip. Within e

How Experion Software Analyzes RNAData PresentationAs the photodiode detects fluorescent signals from the dye-RNA complexes, Experion software convert

Electropherogram generated by Experion RNA HighSens analysis of mRNA. For mRNA assays, thesample peak is usually broad. Any 18S ribosomal RNA contamin

Table of ContentsIntroduction — Product Description and Components ...1The Experion™System and RNA StdSens Starter Kit ...

Virtual gel generated by Experion RNA StdSens analysis of eukaryotic total RNA (scaled to local).Pink triangles indicate the positions of the lower ma

Data Analysis: Normalization, Sizing, and QuantitationFollowing separation, RNA fragments are analyzed using one or more of the following:• An interna

Appendix B — Cleaning the ElectrodesTwo types of cleaning procedures ensure the best results from the Experion™electrophoresisstation: 1) a deep-clean

4. Repeat steps 2 and 3 twice, each time with a fresh swab soaked in DEPC-treatedwater.5. Fill a cleaning chip with 800 µl DEPC-treated water and plac

5. Open the lid and remove the cleaning chip labeled EC.6. Insert the “DEPC” chip. Close the lid for 5 min. Remove the chip and discard the washes.7.

Appendix C — First-Time Use of the Experion™SystemThe first time that the Experion electrophoresis station is used, do the following:1. Establish comm

Appendix D — Ordering InformationCatalog # Description700-7001 Experion™System, 100/120 V, for RNA and DNA analysis, includeselectrophoresis station,

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Life ScienceGroup07-1013 0108 Sig 1207Bulletin 5694 US/EG Rev ABio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 4BIORAD Austra

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Introduction — Product Description and ComponentsThe Experion™System and RNA StdSens Starter KitThe Experion automated electrophoresis system employs

The Experion RNA StdSens starter kit was designed to introduce new users to RNA analysiswith the Experion system as well as to enable troubleshooting,

Kit Components and Storage ConditionsExperion RNA StdSens Starter Kit Components and Storage ConditionsComponent Storage ConditionsExperion RNA StdSen

Experion RNA StdSens Starter Kit TestsTest Name Purpose Description1 RNA analysis basics Used for familiarization Run an Experion RNA StdSens with the

Experion RNA StdSens Starter Kit Test WorkflowEquilibrate the reagentsFilter the gel and prepare the gel-stain solutionPrepare the samples and the RNA