Bio-rad Image Lab™ Software User Manual

Gel Doc™ XR+ and ChemiDoc™ XRS+ Systems with Image Lab™ SoftwareUser GuideVersion 5.1

x | Gel Doc XR+ and ChemiDoc XRS+ with Image Lab Software | Table of Contents

7 | Viewing Images100 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareFit in Window If you zoomed in on an area of an image,

Displaying Gel ImagesUser Guide | 101 The Low progress indicator determines which intensity value is shown at the minimum gray scale (or other co

7 | Viewing Images102 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareImage Colors You can choose a color map for your image

Displaying Gel ImagesUser Guide | 103 False color Spectrum Gold-Silver Pseudo3-D Projection The 3-D view transforms the gel image into a solid

7 | Viewing Images104 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareImage Info The Image Info dialog box provides informati

Displaying DataUser Guide | 105Analysis Table Options Numerical data associated with an analysis can be viewed in an analysis table. Data from the

7 | Viewing Images106 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareDefinitions on page 108. Display — enables you to set

Displaying DataUser Guide | 107Copies the table data to the clipboard so that you can paste the data into word processing or presentation applicat

7 | Viewing Images108 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareBand % — percentage of the band’s volume compared to al

Displaying DataUser Guide | 109Area — area of the volume in mm2.Lane Profile BackgroundBand boundariesThe Lane Profile option shows a cross-sectio

User Guide | 1 1Safety and Regulatory ComplianceImportant Safety InformationPlease read these instructions before attempting to operate the Gel

7 | Viewing Images110 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe zoom tools work the same way they work elsewhere in

Displaying DataUser Guide | 111 Relative Front Volume Absolute Quantity (Abs. Quant.) Relative Quantity (Rel. Quant.) Band % Lane %Adjusting

7 | Viewing Images112 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareStandard Curve The Standard Curve dialog box displays t

Displaying DataUser Guide | 113Report See Generating Reports on page 151, for information about reports.

7 | Viewing Images114 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 1158 Analyzing ImagesAnalysis Tool Box tools are enabled when an image file is opened and in focus. An active or “in focus” window ha

8 | Analyzing Images116 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareAuto Detection SettingsThe band detection sensitivity

Analysis Tool Box ToolsUser Guide | 117Regression Method — four regression methods are available. For more information, see Regression Calculation

8 | Analyzing Images118 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareImage Tools The image tools enable you to manipulate

Image ToolsUser Guide | 119Correcting a Slanted GelTo correct a slanted gel1. In the Rotate section, click Custom. Red arrows appear over the gel

| Safety and Regulatory Compliance12 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareWarrantyThe Gel Doc XR+ or ChemiDoc XRS+

8 | Analyzing Images120 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTo delete crop settings1. With an image open, click C

Lane and Bands ToolsUser Guide | 121Lane and Bands Tools The Lane and Bands tools enable you to identify the lanes and bands in your images. To op

8 | Analyzing Images122 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareDetecting LanesTo detect the lanes in the image, do o

Lane and Bands ToolsUser Guide | 123 Width — changes the width of all lanes at the same time. Click Width and then drag one of the anchor points

8 | Analyzing Images124 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software Bend — bends a lane to better fit the gel image. Cl

Lane and Bands ToolsUser Guide | 125A disk radius that is too large will result in poor background removal. A disk radius that is too small might

8 | Analyzing Images126 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareDetecting BandsBands are detected for individual imag

Lane and Bands ToolsUser Guide | 127Displaying Advanced Detection OptionsYou can also select Display Advanced Options to set specific parameters f

8 | Analyzing Images128 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareAdvanced Detection Options Sensitivity — determines

Lane and Bands ToolsUser Guide | 129 Shoulder — band detection tries to distinguish shoulders as separate bands. When looking at a lane trace, th

Regulatory NoticesUser Guide | 1 3Regulatory NoticesThe Gel Doc XR+ or ChemiDoc XRS+ imaging system is designed and certified to meet EN 61010, t

8 | Analyzing Images130 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software Delete — deletes a band from a lane. Use this featu

Molecular Weight (MW) Analysis ToolsUser Guide | 131Molecular Weight (MW) Analysis Tools Molecular Weight Analysis tools enable you to determine m

8 | Analyzing Images132 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareMolecular Weight StandardYou can change the standards

Molecular Weight (MW) Analysis ToolsUser Guide | 133Standard LanesStandard samples are placed in the first and last lanes by default. You can spec

8 | Analyzing Images134 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe values for the molecular weight in any standard l

Quantity ToolsUser Guide | 135Regression MethodsThere are four regression methods. Linear (semilog) Point-to-point (semilog) Logistic Cubic sp

8 | Analyzing Images136 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software3. Click the band you want to use as a reference. A s

Quantity ToolsUser Guide | 137Absolute Quantity TabAbsolute quantification is used to quantify bands based on known standard bands using a calibra

8 | Analyzing Images138 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe values display in the Standard Bands table. The g

Quantity ToolsUser Guide | 139Linear — generates a straight line that is the best fit of the values you provided and is preferred in most cases. P

| Safety and Regulatory Compliance14 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareNoticeTheGel Doc XR+ or ChemiDoc XRS+ im

8 | Analyzing Images140 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe calibration curve displays. Standards are represe

Annotation ToolsUser Guide | 141Annotation Tools You can annotate results with text and arrows to emphasize areas of interest.

8 | Analyzing Images142 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareAdd Annotations Text — enables you to add text annot

Annotation ToolsUser Guide | 143Text PropertiesYou can change the type size and font of your text annotations.  Font — click the box you want to

8 | Analyzing Images144 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareVolume Tools Volume tools enable you to manually quan

Volume ToolsUser Guide | 145A default label appears within the shape drawn. The volume label is assigned a sequential number and can be one of thr

8 | Analyzing Images146 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareVolume TypesYou can define the volume type (unknown,

Volume ToolsUser Guide | 147Volume Background SubtractionWhen you draw a volume, some nondata background pixels might be included inside the volum

8 | Analyzing Images148 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software If you create more than one background volume, all

Volume ToolsUser Guide | 149Standard volumes will have the default names S1, S2, and so on, based on the sequence of their creation. To review the

Power Safety InformationUser Guide | 1 5Power Safety InformationVoltage Setting InformationThe universal hood of the Gel Doc XR+ or ChemiDoc XRS+

8 | Analyzing Images150 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 1519 Generating ReportsAfter viewing results, you can generate a report that displays the analyzed gel images, all of the settings us

9 | Generating Reports152 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareReport Settings Use this dialog box to customize th

ReportUser Guide | 153When you select this checkbox, both the annotated image (if available) and unannotated images are printed in the report. The

9 | Generating Reports154 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareLane and Band Table TabThe Lane and Band Table tab

ReportUser Guide | 155Volume Table TabThe Volume Table tab includes the following settings: Include Volume Table — clear to exclude this informat

9 | Generating Reports156 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareAdjust the Printer Settings The Printer Settings bu

User Guide | 15710 Exporting ResultsThe most convenient way to archive complete information about experiments is to produce reports. However, you

10 | Exporting Results158 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe options to export gel images are available usin

Exporting Gel ImagesUser Guide | 159In this dialog box you can: Select the entire image or the current view Select the resolution or specify a c

| Safety and Regulatory Compliance16 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

10 | Exporting Results160 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareYou can zoom in on an area in a current view and ex

Exporting Gel ImagesUser Guide | 161Image Lab reduces the image to an 8-bit .tif image file. Resolution is limited and file size is restricted to

10 | Exporting Results162 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareCopy Analysis Table to the Clipboard Copies the ana

User Guide | 16311 System CalibrationWhen your imager is installed, instrument calibration is performed with a calibration wizard. See the install

11 | System Calibration164 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareRecalibrating Your ImagerWhen you add light source

Recalibrating Your ImagerUser Guide | 1652. The software prompts you to restart the calibrations needed for the new illumination sources.3. Click

11 | System Calibration166 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 16712 Image Lab LogsImage Lab LogsImage Lab™ software provides three types of logs. Instrument log — records events related to the i

12 | Image Lab Logs168 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareViewing the Instrument LogInstrument operations that y

Image Lab LogsUser Guide | 169Viewing the System LogEvents related to running Image Lab, including enabling or disabling of secure mode and users

User Guide | 1 71 IntroductionGel Doc™ XR+ and ChemiDoc™ XRS+ gel documentation systems offer high performance and ease of use. They both contain

12 | Image Lab Logs170 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareViewing the Document LogThe View menu displays a list

Image Lab LogsUser Guide | 171To open the Document Log Viewer From the View menu, click the document log that you want to view. A typical documen

12 | Image Lab Logs172 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTo display or hide columns1. From the View menu, open

Image Lab LogsUser Guide | 173Setting filters in logsTo filter the entries of a column1. In the log, click the heading of the column that you want

12 | Image Lab Logs174 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareRemoving filters in logsThe Remove all filters icon is

Image Lab LogsUser Guide | 175To display the full content of a row Click the Collapse or expand rows icon to expand the row height. The row adjus

12 | Image Lab Logs176 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTo export a log file1. From the View menu, click the l

Image Lab LogsUser Guide | 177The Log Print Preview window displays the contents of the log file.From the Log Print Preview window, you can: Clic

12 | Image Lab Logs178 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 17913 Using the Security Edition21 CFR Part 11Image Lab™ Security Edition is a module within the Bio-Rad Image Lab software that assi

1 | Introduction18 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software Smart, application-based protocol setup using Image Lab

13 | Using the Security Edition180 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareStandard Mode versus Secure ModeImage La

User Names, Groups, and RolesUser Guide | 181There are four default roles in Image Lab Security Edition. Each role is associated with one of the f

13 | Using the Security Edition182 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareRole RestrictionsYour role determines wh

Starting Image Lab Security EditionUser Guide | 183Starting Image Lab Security EditionWhen secure mode is enabled, you are prompted to log on when

13 | Using the Security Edition184 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareUnsecured DocumentsProtocol and image fi

Electronic RecordsUser Guide | 185Documents created in secure mode can be signed at any time. When a secure document is signed, it is saved as rea

13 | Using the Security Edition186 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareSigning DocumentsTo sign a document1. Se

Document LogsUser Guide | 1876. Enter a new name for the file and click Save.Note: Signed protocols are saved with an .sptl extension, signed ima

13 | Using the Security Edition188 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareViewing the Document LogThe View menu di

User Guide | 189A MaintenanceThis chapter includes instructions for maintaining the universal hood in proper working condition by replacing parts.

System ComponentsUser Guide | 1 9A patent-pending software algorithm controls the MZL, giving the user automatic image focus once an initial calib

A | Maintenance190 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software5. Place it on a nonabrasive surface so that the glass doe

Epi-Illumination Lamp ReplacementUser Guide | 191Epi-Illumination Lamp ReplacementThe lamps are located behind two cover panels on the left and ri

A | Maintenance192 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareFuse ReplacementCaution: Always unplug the instrument bef

User Guide | 193B TroubleshootingFollow these suggestions to troubleshoot your system.Problem Possible Cause SolutionCamera does not respond/camer

B | Troubleshooting194 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwarePrintout does not look like the monitor image The mon

User Guide | 195C Setting Up Users and GroupsSetting Up Image Lab Users and Groups This appendix explains how to set up users and groups to run Im

C | Setting Up Users and Groups196 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareUser AuthenticationIn the user authentic

This checkbox specifies whether valid users and groups are defined on (1) the local Image Lab computer, or (2) on the network domain.Setting Up Imag

C | Setting Up Users and Groups198 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTo find the name of your network domain1

Setting Up Image Lab Users and GroupsUser Guide | 199To create a new user on a local computer1. Click on the Users folder to open it and select Ac

NoticeNo part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recordi

1 | Introduction20 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareEmission FiltersThe universal hood can hold two different

C | Setting Up Users and Groups200 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Softwarepassword before using Image Lab Security

Setting Up Image Lab Users and GroupsUser Guide | 2012. Click Advanced to expand the dialog box.3. In the expanded dialog box, click Find Now to p

C | Setting Up Users and Groups202 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareConfiguring Users and Groups on a Networ

Setting Up Image Lab Users and GroupsUser Guide | 2032. Fill out all the fields: User name — The user name must be unique.  Full name — The full

C | Setting Up Users and Groups204 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTo create a new group on a Windows serve

Setting Up Image Lab Users and GroupsUser Guide | 2052. Click Advanced to expand the dialog box.3. In the expanded dialog box, click Find Now to p

C | Setting Up Users and Groups206 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwarePassword Security21 CFR 11.300 (b) requi

Password SecurityUser Guide | 2073. To change password policy settings:a. Right-click the policy and select Properties to open its properties dial

C | Setting Up Users and Groups208 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwarePassword Policy Setting ExamplesThe foll

Miscellaneous Security MeasuresUser Guide | 2093. In the section When maximum event log size is reached: select Do not overwrite events (Clear log

User Guide | 2 12 Gel Doc XR+ ImagerRefer to the Gel Doc™ XR+ Installation Guide found in your Gel Doc XR+ Installation Kit for information about

C | Setting Up Users and Groups210 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 211D AccessoriesCalibrating AccessoriesIf you are installing accessories along with your original system installation, calibrate your

D | Accessories212 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareXcitaBlue™ Conversion ScreenThe optional XcitaBlue convers

Installing Optional AccessoriesUser Guide | 213The XcitaBlue conversion screen is held in place by adhesive-backed edge guides. After the edge gui

D | Accessories214 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareGel Alignment Template KitThe Bio-Rad gel alignment templa

Installing Optional AccessoriesUser Guide | 215To install and use the gel alignment template kit1. Place the locator frame over the transilluminat

D | Accessories216 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software4. Place the white light transilluminator on top of the UV

Installing Optional AccessoriesUser Guide | 217 Krypton Qdot 625The orange fluorescence reference plate (catalog #170-8008) can be used with sev

D | Accessories218 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareCalibrating the Imager to Use the Orange Fluorescence Refe

Ordering InformationUser Guide | 219170-8265 ChemiDoc XRS+ with Image Lab software system for PC/Mac Installation Kits170-8199 Image Lab™ Upgrade

2 | Gel Doc XR+ Imager22 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareGel Doc XR+ ApplicationsThe Gel Doc XR+ system is

D | Accessories220 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software170-8081 Standard emission filter replacement, 62 mm100-13

Ordering InformationUser Guide | 221Nucleic Acid Standards 170-8351 EZ Load™ 20 base pairs molecular ruler170-8352 EZ Load 100 base pairs molecula

D | Accessories222 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 223E Using Bio-Rad’s Stain-Free TechnologyBio-Rad’s stain-free gels eliminate the time-consuming staining and destaining steps requir

E | Using Bio-Rad’s Stain-Free Technology224 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe benefits of stain-free tec

Electrophoresis with Stain-Free GelsUser Guide | 225Electrophoresis with Stain-Free GelsStain-free gels are made and packaged without sodium dodec

E | Using Bio-Rad’s Stain-Free Technology226 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareImaging BlotsTo blot stain-fre

User Guide | 227F Mitsubishi P93/P95 Thermal PrinterSetting up a Thermal Printer on WindowsThe printer driver is on the Image Lab™ software insta

F | Mitsubishi P93/P95 Thermal Printer228 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareSetting up a Thermal Printer on a M

User Guide | 229G Regression Calculation MethodsEach regression method calculates a standard curve. Some of the methods provide the formula for th

Gel Doc XR+ Technical SpecificationsUser Guide | 2 3Gel Doc XR+ Technical Specifications ApplicationsChemiluminescence NoFluorescence* YesColorime

G | Regression Calculation Methods230 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Softwarewhere:x = mobilityy = molecular weighta

User Guide | 231Glossary Aspect ratio The ratio of the width to the height of an image.CCD (Charge-coupled device) A light-sensitive silicon chip

| Glossary232 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

Life ScienceGroup13-2297 1213 Sig 121310017218 Rev G US/EG Bio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia

2 | Gel Doc XR+ Imager24 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareEmission filters  1 included (standard) 3 option

User Guide | 2 53 ChemiDoc XRS+ ImagerRefer to the ChemiDoc™ XRS+ Installation Guide found in your Image Lab™ upgrade for the ChemiDoc XRS+ system

3 | ChemiDoc XRS+ Imager26 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareChemiDoc XRS+ ApplicationsThe ChemiDoc XRS+ system

ChemiDoc XRS+ Technical SpecificationsUser Guide | 2 7ChemiDoc XRS+ Technical Specifications ApplicationsChemiluminescence YesFluorescence* YesCol

3 | ChemiDoc XRS+ Imager28 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareEmission filters  1 included (standard) 3 option

User Guide | 2 94 Setting Up the Instrument with Image Lab SoftwareSystem RequirementsImage Lab™ software runs on Microsoft Windows XP Professiona

User Guide | iiiTable of ContentsSafety and Regulatory Compliance. . . . . . . . . . . . . . . . . . . . . . . . . .11Important Safety Information

4 | Setting Up the Instrument with Image Lab Software30 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareInstalling Image La

Installing Image Lab SoftwareUser Guide | 3 1The Image Lab installer automatically launches.2. On the Welcome screen, click Next.3. Accept the lic

4 | Setting Up the Instrument with Image Lab Software32 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareNote the following.

Installing Image Lab SoftwareUser Guide | 3 36. A screen appears requesting your license code.Note: Your 18-digit license code can be found in th

4 | Setting Up the Instrument with Image Lab Software34 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software10. On the Ready to

Installing Image Lab SoftwareUser Guide | 3 511. When the installation is complete, you are prompted to display the Release Notes and/or the Windo

4 | Setting Up the Instrument with Image Lab Software36 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe Image Lab icon

Installing the Drivers on a Windows XP ComputerUser Guide | 3 7The dialog box listing the driver that will be installed appears. 2. By default, th

4 | Setting Up the Instrument with Image Lab Software38 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareWindows installs th

Installing the Drivers on a Windows 7 ComputerUser Guide | 3 9Installing the Drivers on a Windows 7 ComputerIf you are running Windows 7, the devi

iv | Gel Doc XR+ and ChemiDoc XRS+ with Image Lab Software | Table of ContentsChapter 2 Gel Doc XR+ Imager . . . . . . . . . . . . . . . .

4 | Setting Up the Instrument with Image Lab Software40 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe License Code fi

Setting Up Image Lab Security EditionUser Guide | 4 1Within about 30 seconds you will receive a confirmation that your Image Lab Security Edition

4 | Setting Up the Instrument with Image Lab Software42 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareA Save File window

Setting Up Image Lab Security EditionUser Guide | 4 39. Select Activate Via Receive Activation Email.10. Click Receive Email.An Open File window a

4 | Setting Up the Instrument with Image Lab Software44 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe Security Editio

Setting Up Image Lab Security EditionUser Guide | 4 5The system sends a message to the Bio-Rad Technical Support Department, a window stating that

4 | Setting Up the Instrument with Image Lab Software46 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareA Save File window

Setting Up Image Lab Security EditionUser Guide | 4 7To enable secure mode1. From the Security menu, click Security Preferences to display the Sec

4 | Setting Up the Instrument with Image Lab Software48 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareA message appears s

Setting Up Image Lab Security EditionUser Guide | 4 9The Admin Authentication dialog appears.4. Enter your Image Lab Administrator user name and p

User Guide | v | Table of ContentsChapter 6 Acquiring Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69The Protocol

4 | Setting Up the Instrument with Image Lab Software50 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareA message appears s

Setting Up Image Lab Security EditionUser Guide | 5 1 In the Domain to be used in authentication field, enter the name of your network domain. Se

4 | Setting Up the Instrument with Image Lab Software52 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software Select the Use lo

Setting Up Image Lab Security EditionUser Guide | 5 3Renaming Security GroupsTo rename any of the four default Security Groups1. From the main men

4 | Setting Up the Instrument with Image Lab Software54 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareFor information abo

User Guide | 5 55 Image Lab Software OverviewImage Lab™ image acquisition and analysis software works with the Gel Doc™ XR+ and ChemiDoc™ XRS+ im

5 | Image Lab Software Overview56 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareTable 2 lists the extensions and icons f

Interface OverviewUser Guide | 5 7 Interface OverviewThe following illustration shows the Image Lab main window. This section explains the main so

5 | Image Lab Software Overview58 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareMain ToolbarMany Image Lab tools can be s

Interface OverviewUser Guide | 5 9Start PageThe Start Page guides you through creating, opening, and viewing protocols and images.Analysis Tool Bo

vi | Gel Doc XR+ and ChemiDoc XRS+ with Image Lab Software | Table of ContentsChapter 8 Analyzing Images . . . . . . . . . . . . . . . . .

5 | Image Lab Software Overview60 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareAn image file must be selected to make an

Interface OverviewUser Guide | 6 1MW Analysis Tools (Molecular Weight Analysis) enables you to choose standard samples, assign standard lanes, and

5 | Image Lab Software Overview62 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareMenu CommandsThe following section descri

Interface OverviewUser Guide | 6 3 Export for PulseNet — reduces the image to an 8-bit .tif file. Resolution is limited and file size is restrict

5 | Image Lab Software Overview64 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareEdit Menu CommandsUndo undoes the last ac

Interface OverviewUser Guide | 6 5 The Protocol tab shows presets for naming image files. You can choose to include a designated Prefix, User Nam

5 | Image Lab Software Overview66 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareSecurity Menu CommandsNote: This menu is

Interface OverviewUser Guide | 6 7Imitate Transform changes the brightness and contrast of all open images to the same transform settings as the c

5 | Image Lab Software Overview68 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 6 96 Acquiring ImagesImage Lab software runs specific applications with repeatable workflows using configurable protocols that have

User Guide | vii | Table of ContentsChapter 10 Exporting Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157Exporting Ge

6 | Acquiring Images70 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe Protocol Setup WindowThe left pane displays headin

Creating a ProtocolUser Guide | 7 1You can review protocol settings by selecting Protocol Summary, which lists the settings for each step in the r

6 | Acquiring Images72 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareSetting Up a New ProtocolThe following steps set up (o

Creating a ProtocolUser Guide | 7 3Step 1. Gel Imaging for Single-Channel ProtocolsTo take an image of the gel or blot, you need to configure the

6 | Acquiring Images74 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareChoose Custom to save and run an existing application

Creating a ProtocolUser Guide | 7 5 Manual Exposure — use this setting to manually override automated imaging. Exposure time can range from 0.001

6 | Acquiring Images76 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareRather than manually acquiring a series of independent

Creating a ProtocolUser Guide | 7 7Although SAM is useful for determining the optimum imaging time for a chemiluminescent sample, it results in da

6 | Acquiring Images78 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareStep 2. Detect Lanes and BandsTo analyze the gel or bl

Creating a ProtocolUser Guide | 7 9 Custom — select a numeric value from 1 to 100 to choose the best detection sensitivity for your sample.Step 3

viii | Gel Doc XR+ and ChemiDoc XRS+ with Image Lab Software | Table of ContentsModifying Secure Documents . . . . . . . . . . . . . . . . .

6 | Acquiring Images80 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Softwarea. Click Change to display the Manage Standards dialog

Creating a ProtocolUser Guide | 8 1a. Enter a name for the new standard.b. Choose a standard type: protein or nucleic acidA protein standard can b

6 | Acquiring Images82 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software Gradient gels — the linear (semilog) regression meth

Creating a ProtocolUser Guide | 8 3 Automatically print a report Display the reportImage Lab prints to the default printer unless you select oth

6 | Acquiring Images84 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareCreating a Multichannel Image from Single ImagesYou ca

Editing a Saved ProtocolUser Guide | 8 5Editing a Saved ProtocolYou can change the protocol settings and rename the protocol using Image Lab tools

6 | Acquiring Images86 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareYou see the same set of menus and choices described in

Positioning the GelUser Guide | 8 7Positioning the GelTo position a gel1. Click the yellow Position Your Gel button in the Protocol Setup window.2

6 | Acquiring Images88 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareRunning a ProtocolTo run a protocol Click Run Protoco

Running Signal Accumulation Mode (SAM) ProtocolsUser Guide | 8 9Running Signal Accumulation Mode (SAM) ProtocolsYou can interrupt the acquisition

User Guide | i x | Table of ContentsAppendix E Using Bio-Rad’s Stain-Free Technology . . . . . . . . . 223Stain-Free Workflow . . . . . . . .

6 | Acquiring Images90 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareSaving Signal Accumulation Mode (SAM) ImagesYou can sa

Setting Up a Custom ApplicationUser Guide | 9 1Setting Up a Custom ApplicationUse the Manage Custom Application dialog box to save an existing app

6 | Acquiring Images92 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareThe Create Custom Application dialog box appears. 3. E

Regression MethodsUser Guide | 9 3Regression MethodsA regression method is used to calculate the molecular weight of the unknown bands. The softwa

6 | Acquiring Images94 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareApplication TablesThe following tables specify the lig

Application TablesUser Guide | 9 5Silver stain White trans None Standard None White convLensCoomassie Fluor OrangeXcitaBlue UV 560/50 None Orange

6 | Acquiring Images96 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software

User Guide | 9 77 Viewing ImagesAfter a gel is imaged, the image appears in the workspace. Many controls are available to optimize viewing and to

7 | Viewing Images98 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab SoftwareDisplaying Gel Images The display toolbar is located abo

Displaying Gel ImagesUser Guide | 9 9Band AttributesYou can show the following attributes for selected lanes or for all lanes. Band number Band