Bio-rad DCode™ Universal Mutation Detection System User Manual

WinMelt™and MacMelt™DNA Melting Profile Analysis SoftwareVersion 2.0Catalog NumbersWinMelt 170-9240MacMelt 170-9034For Technical Service Call Your Loc

should only take a fraction of a second. However, if the sequence is long or if the computeris not fast, a delay may be noticed.Fig. 3.6. Project View

To reset the graph to the default settings, select Default Graph Axis from the Graphmenu. WinMelt users can also click on the right mouse button. The

Section 4Using WinMelt/MacMelt for Denaturing Gradient GelElectrophoresisCalculating a melt profile for a wild-type or known sequence using the WinMel

Fig. 4.1. A 299 base-pair betaglobin sequence (exon 1).Figure 4.2 shows the melting profile for the betaglobin sequence graphed using the 50%melted pr

Fig. 4.3. The example betaglobin sequence with a 40 bp GC clamp attached to the 3’ end.Figure 4.4 shows the melting profile for the 339 base-pair GC c

Fig. 4.5. The melting profiles for a mutant and wild-type sequence from a portion of the beta-globin gene (exon 1).Sometimes the addition of a GC-clam

Section 5The Project ViewThe Project View is a window that shows an overview of the project file (Figure 5.1).The name of the project is shown in the

When a project is saved, all sequences, sequence descriptions, and options are stored inthe project file. The actual melting profiles are not stored i

Section 7The Graph ViewThe Graph View shows the melting profile graphs of the sequences in the project thatwere selected to be graphed (Figure 7.1). A

Fig. 7.2. Edit Graph Axis window.The graph can be saved as an image file for importing into another application. To savethe graph, select Export from

WarrantyBio-Rad Laboratories warrants that the MacMelt and WinMelt software shall substan-tially conform, in all operational features, to Bio-Rad&apos

Fig. 7.3. Printed graph and sequence information.17DNA melting profile graphBetaglobinSequence position (bp)Betaglobin exon 1 with GC-clampMelting pro

18Section 8Menu Descriptions8.1 File MenuThe File menu contains command for creating, opening, saving, and printing projects.The command for exiting

PrintThe Print command will print the contents of the active window. The command will open thestandard Print dialog box and allow selection of printin

CopyThe Copy command copies the selected text to the clipboard for later use. The selected textis not deleted. The text on the clipboard can later be

DeleteThe Delete command will delete the currently selected sequence from the project.DuplicateThe Duplicate command will make an identical copy of th

8.5 View MenuToolbarThe Toolbar command will show or hide the toolbar. A tick mark will appear on the menuwhen the toolbar is shown. The toolbar can

More Windows...If there are many open windows on the screen, the More Windows command will appear.Choosing this command will show a list of all the wi

CutThe Cut button deletes the selected text and places the deleted text on the clipboardfor later use. The text on the clipboard can later be inserted

Show or Hide GraphThe Show or Hide Graph button will show or hide the Graph View.Edit Graph AxisThe Edit Graph Axis button allows the axis of the grap

Section 11References1. Lerman, L. S. and Silverstein K., Methods Enzymol., 155, 482–501 (1987). 2. Sheffield, V. C., Cox, R. D., Lerman, L. S. and Mye

Table of ContentsSection 1 Installation ...11.1 Abo

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Section 1Installation1.1 About this ManualThis manual is designed for both the Windows and Macintosh version of the software. Thetwo programs, WinMel

ware on two disks for your convenience. Follow this procedure if installation of Win32s isrequired for your computer.1. Start Microsoft Windows.2. Ins

Section 2Program OverviewThe thermodynamics of the transition of double-stranded to single-stranded DNA havebeen described by the computer program Mel

Section 3Quick TutorialThis tutorial section allows you to work with the main features of the program. Thetutorial will cover.• How to start the progr

A sequence can be added to the project by two methods: 1) The sequence can be import-ed from a text file, or 2) the entire sequence can be typed into

Fig. 3.4. Sequence Display window for the Copy of TP53ex8 wild-type sequence.Introduce a "G to A" mutation at position 47 by selecting the &