Bio-rad Gene Pulser MXcell™ Electroporation System User Manual

Gene TransferGene Pulser MXcell™Electroporation Guide

Electroporation Conditions Are KnownCell number <5 x 106Gene Pulser MXcell Electroporation Guide Check all well sets after each electroporation for

11Electroporation Conditions Are KnownCell number >5 x 106Gene Pulser MXcell Electroporation Guide Check all well sets after each electroporation f

Electroporation Conditions Are KnownCell number >5 x 106Gene Pulser MXcell Electroporation Guide Check all well sets after each electroporation for

13Exponential and Square Waveforms ProtocolsUse preset protocols Uniform 96-well/Exp, Sqr to identify optimal conditions for the different cell lines

Once the best waveform is identified, use this tree to program the MXcell system to improve other parameters.Electroporation Protocols Decision TreeGen

15Electroporation ProtocolGene Pulser MXcell Electroporation Guide Visit us on the Web at discover.bio-rad.com | Electroporation ProtocolMaterials

There are many techniques available to examine cells for transfection efficiency and cell viability. We have briefly summarized three commonly used meth

17Fluorometer and Scanner Analysis for Adherent CellsFluorometric analysis of cell lysates can be used to examine lysed cells for the presence of a fl

96-well/Exp 96 96 Use for initial optimal protocol Applies the same waveform identification for many cell types across the whole plate96-w

Life ScienceGroup08-0394 0608 Sig 0308Bulletin 5700 Rev A US/EGBio-RadLaboratories, Inc. Web site www.bio-rad.com USA 800 4BIORADAustralia 6

2To choose a starting set of protocols, search our library of electroprotocols at www.bio-rad.com/genetransferprotocols/ or search http://www.ncbi.n

3Electroporation plate: a plate in which electroporation is performed; available in 12-, 24-, and 96-well formatsMini protocol: a preprogrammed set o

Electroporation Parameters Selection PathwaysGene Pulser MXcell Electroporation Guide Identify Pulse WaveformExponential Waveform Square WaveformOptim

5Go to page 7Known electroporation conditionsYou know the electroporation conditions or found a suitable set of protocols in our libraryYou need a qu

Go to pages 9–10Go to pages 11–12Cell number <5 x 106Use Mini ProtocolsCell number >5 x 106Use Whole Plate ProtocolsMultiple cell linesUse Whol

7Go to page 8Go to page 8Cell number <5 x 106Use Mini ProtocolsCell number >5 x 106Use Whole Plate ProtocolsCell numberBased on the number of c

Cell number >5 x 106Use preset protocols Opt 96-well/Exp, Sqr to identify waveforms and other initial conditions.Gene Pulser MXcell Electroporatio

9Electroporation Conditions Are KnownCell number <5 x 106 Exponential Waveform ProtocolsUse preset protocols Opt mini 96-well/Exp to identify opti