Bio-rad Biotinylated Standards User Manual download pdf

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

LIT395 Rev D

Biotinylated SDS-PAGE

Standards, Low, High,

and Broad Range

Bio-Rad’s Biotinylated SDS-PAGE Standards are

a mixture of biotinylated proteins that can be used for

accurate molecular weight determinations of immune

detected proteins. The standards have been blended to

give equal intensities when detected with avidin-HRP

and HRP Color Development Reagent.

The Biotinylated SDS-PAGE Standards and the

sample proteins are run on an SDS polyacrylamide gel

and electrophoretically transferred to nitrocellulose,

PVDF, or Zeta-Probe

membranes. The protein sam-

ples and standards are exposed to the same reagents

during detection. This prevents distortions that may

occur if the standards lane is treated separately.

The molecular weights of the standards are not sig-

nificantly altered by biotinylation. The consistent

molecular weights of the standards give accurate

molecular weight determinations every time without

increasing the number of steps or length of time of

immune detection.

Specifications

MW Ranges Low 14,400–97,000 daltons

High 45,000–200,000 daltons

Broad 6,500–200,000 daltons

Low High Broad

Protein MW (daltons) Range Range Range

Myosin 200,000 X X

β-galactosidase 116,250 X X

Phosphorylase b 97,400 X X X

Bovine serum albumin 66,200 X X X

Ovalbumin 45,000 X X X

Carbonic anhydrase 31,000 X X

Soybean trypsin inhibitor 21,500 X X

Lysozyme 14,400 X X

Aprotinin 6,500 X

Contents Approximately 130 µg total protein in a buffer

of 50% glycerol, 150 mM NaCl, 3 mM NaN

Volume 250 µl

Storage -20 °C

Shelf life 1 year at -20 °C

Applications Dilute 1:4 for HRP color development; 60–100

applications per vial

Dilute 1:20 for AP color development; 300–500

applications per vial

Ordering Information

Catalog

Number Product Description

161-0319 Biotinylated SDS-PAGE Standards, Broad Range,250 µl

161-0306 Biotinylated SDS-PAGE Standards, Low Range, 250 µl

161-0311 Biotinylated SDS-PAGE Standards, High Range, 250 µl

161-0322 Biotinylated SDS-PAGE Standards Kit, Broad Range, AP*

161-0321 Biotinylated SDS-PAGE Standards Kit, Broad Range, HRP*

161-0307 Biotinylated SDS-PAGE Standards Kit, Low Range, HRP*

161-0308 Biotinylated SDS-PAGE Standards Kit, Low Range, AP*

161-0312 Biotinylated SDS-PAGE Standards Kit, High Range, HRP*

161-0313 Biotinylated SDS-PAGE Standards Kit, High Range, AP*

170-6528 Avidin-HRP, 2 ml

170-6533 Avidin-AP, 1 ml

170-6431 Horseradish Peroxidase Conjugate Substrate Kit

170-6432 Alkaline Phosphatase Conjugate Substrate Kit

* Each kit contains 250 µl Biotinylated Standards, 1 ml

Avidin-AP or 2 ml Avidin-HRP, and complete instructions.

Biotinylated SDS-PAGE

Standards, Low, High,

and Broad Range

Catalog Numbers

161-0306

161-0311

161-0319

LIT395D 9/3/98 9:39 AM Page 1

1 2 3

Summary of Contents

Page 1 - Standards, Low, High

12Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT395 Rev DBiotinylated SDS-PAGEStandards, Low, High,and Broad RangeBio-Rad’s Biotin

Page 2

12Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547LIT395 Rev DBiotinylated SDS-PAGEStandards, Low, High,and Broad RangeBio-Rad’s Biotin

Page 3 - Protocol

ABFig. 1. Biotinylated SDS-PAGE Standards, low andbroad range. A. Low range biotinylated SDS-PAGE stan-dard run on a 12% gel, blotted to nitrocellulos

Bio-rad Biotinylated Standards User Manual

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