Bio-rad PROTEAN Plus Dodeca Cell User Manual

PROTEAN Plus™ Dodeca™CellInstruction ManualCatalog Numbers165-4150165-4151For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-8

Standard running buffer formulations:Tris/Glycine/SDS25mM Tris-Base (M.W. 121.1), 192mM Glycine (M.W. 75.07), 0.1% SDS, pH 8.3(Do not adjust the pH wi

3.2.2 PROTEAN Plus gel cassette preparationCast gels in the PROTEAN Plus hinged spacer plates as directed in the PROTEAN PlusMulti-Casting Chamber an

disturb the protein resolution pattern and need to be removed. Immediately after overlaying, usethe spatula and tap on top of the strip against the pl

When running PROTEAN II Ready Gels or 20 x 20 cm PROTEAN II gels, install theeleven pieces of manifold tubing onto the black fittings in manifold in t

3.5 Starting the electrophoresis runBe sure to check the following:• The gel is placed in the tank with the hinged side down. If you are using unhing

Pick up the gel by gently lifting the bottom of the gel first with two hands and thenslowly continue lifting upward until the gel is completely off th

Section 5Troubleshooting GuideProblem Cause Solution1. Smile effect– band pattern a. Center of the gel running a. Improper cooling.curves upward at bo

Problem Cause Solution5. SDS is precipitating. a. Running buffer is too cold. a. Set the refrigerated circulator to maintain a buffer temperature of a

PROTEAN Plus Multi-Casting ChamberCatalogNumber Description165-4160 PROTEAN Plus Multi-Casting Chamber, includes chamber,sealing plate, silicone gaske

Power supplyCatalogNumber Description165-5052 PowerPac 200 power supply, 100/120 V165-5053 PowerPac 200 power supply, 220/240 V165-5062 PowerPac shelf

Table of ContentsPageSection 1 General Information...11.1 Intro

Life ScienceGroup Sig 0603Bio-RadLaboratories, Inc.Web siteUSA(800) 4BIORAD Australia02 9914 2800 Austria(01)-877 89 01 Belgium09-385 55 11 Braz

Section 1General Information1.1 IntroductionThe PROTEAN Plus Dodeca Cell* is a multi-cell for polyacrylamide gel electrophoresis.It is primarily inte

2Specifications of the Buffer Recirculation PumpNumber of channels 1Flow rate range 0 - 1.5 liters/min, as measured with 3/8" tubing andwater. F

Section 2Setting up PROTEAN Plus Dodeca Cell for the first time2.1 Unpack the cellThe complete PROTEAN Plus Dodeca Cell arrives in two boxes: The tan

Ceramic cooling core Coolant from a refrigerated circulator passesthrough the ceramic cooling core to cool thebuffer during the electrophoresis run. A

5PROTEAN Plus hinged spacer plate The hinged spacer plates close to form a gelcassette. The hinge is necessary to seal the bottom edge of the gel cass

Prior to connecting the power cord to the power entry module and wall outlet, verify that thevoltage indicated on the power entry module matches your

Note: Do not turn on the pump. Wait until the lid is attached to the cell, and you are ready tostart the run.2.4 Additional required components Refr