Bio-rad Biotinylated Standards User Manual

Biotinylated StandardsKitCatalog Numbers161-0307161-0308161-0312161-0313161-0321161-03224006050C 5/24/99 8:35 AM Page i

Related Accessories (continued)Catalog Number Product Description170-6425 Protein G HRP, 1 ml170-6426 Protein G Gold, 2 ml170-6527 Colloidal Gold Tota

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 945474006050 Rev C 4006050C 5/24/99 8:36 AM Page 16

Table of ContentsSection 1 Introduction ... 1Section 2 Specifications ...

Section 1IntroductionBio-Rad's biotinylated standards are biotinylated proteins that can beused for accurate molecular weight determinations of i

Section 2SpecificationsTable 1 Composition of the Biotinylated SDS-PAGEStandardsMWProtein Source (dalton) High Low Broad ReferenceMyosin rabbit skelet

Section 4Solutions4.1 Solutions for Electrophoresis Gels1. Sample buffer (SDS-PAGE reducing buffer)Distilled water 4.0 ml0.5 M Tris-HCl pH 6.8 1.0 mlG

Section 5Assay Procedure5.1 General Recommendations1. Solution volume. The liquid in the incubation vessel should be at least0.25 cm deep to insure th

Section 6Troubleshooting Guide6.1 Activity Test for ReagentsA. Activity test for HRP color development solution.Combine 1.0 ml of the color developmen

6.4 No Color in Sample, Good Color Development inStandardsFor problems with immune detection of the samples, consult Bio-Rad'sImmun-Blot assay ki

Section 8Ordering InformationCatalog Number Product Description161-0306 Biotinylated SDS-PAGE Standards, Low Range, 250 µl161-0307 Biotinylated SDS-PA