Bio-rad PROTEAN® Plus Hinged Spacer Plates and Combs User Manual

Model 385 and 395Gradient FormerInstruction ManualCatalog Numbers165-2000 and 165-2001For Technical Service Call Your Local Bio-Rad Office or in the U

Fig. 7.1. Linear gradient gel. a. 5-30% gradient. b. 10-20% gradient.Fig. 7.2. Concave gradient—relation of heavy and light solutions in the mixing an

Fig. 7.3. Concave gradient gel.Fig. 7.4. Convex gradient—relation of light and heavy solutions in the mixing and reservoir cham-bers. Intensity of sha

Fig. 7.5. Convex gradient gel.Section 8Protocols8.1 Preparation of Stock Solutions for SDS-PAGE Slab Gels(Laemmli* buffer system)A. Acrylamide/Bis (30

F. Sample Buffer (SDS reducing buffer: 62.5 mM Tris-HCl, pH 6.8, 10% glycerol,2% SDS, 5% b-mercaptoethanol)Distilled water 4.0 ml0.5 M Tris-HCl pH 6.8

b. The total volume required for multiple gels can be determined by multiplying the vol-ume of a single gel by the number of gels required.8.3 Gel Pre

8.4 Linear Gradient GelsFor linear gradients, the volume in the mixing chamber (Vm) equals the volume in thereservoir chamber (Vr). Try volume in each

8.5 Exponential Gradient GelsFor an exponential gradient gel, the volume in the mixing chamber is one fourth the totalrequired gel volume (0.25 VT) an

Section 9Equipment and Accessories9.1 Model 385 and Model 395 Gradient Formers and AccessoriesCatalogProduct Description NumberModel 385 Gradient Form

CatalogProduct Description NumberPROTEAN II xi 2-D Cell, 1.0 mm, 16 cm 165-1931PROTEAN II xi 2-D Cell, 1.5 mm, 16 cm 165-1932PROTEAN II xi 2-D Cell, 1

CatalogProduct Description NumberMini-PROTEAN II Slab Cell, 10 well combs, 0.75 mm spacers, 165-2940includes inner cooling core with gaskets, lower bu

Table of ContentsPageSection 1 Introduction...11.1 Specificati

CatalogProduct Description NumberSDS (sodium dodecylsulfate) 25 g 161-0300100 g 161-03011 kg 161-0302Boric Acid 500 g 161-07501 kg 161-0751Ammonium Pe

Life ScienceGroupBio-Rad Laboratories Main Office 2000 Alfred Nobel Drive, Hercules, California 94547, Ph. (510) 741-1000, Fx. (510)741-5800Also in: A

Section 1IntroductionThe Model 385 and Model 395 Gradient Formers are primarily used to construct repro-ducible linear and exponential polyacrylamide

1.1 SpecificationsModel 385 Gradient FormerOverall size 10 cm x 15 cm x 15 cm (with piston in chamber)Weight 405 g without piston; piston 65 gCapacity

If a peristaltic pump is not used, the level of the gradient former stopcock must be abovethe top of the gel sandwich by a distance sufficient to crea

2. Insert the needle into the sandwich through the gasket on the bottom of the casting stand.3. To start the linear gradient, use the procedure descri

6. Quickly turn on the stirring motor (to a speed which gives good mixing without foaming),open the valve stem and the stopcock, and start the perista

Section 5Pouring Multiple Gradient Gels in a Gel Casting ChamberNote: Gel casting chambers are designed for bottom filling, therefore concave exponen-

Section 6Gradient Former Care and MaintenanceAfter use, disassemble the gradient former and rinse all parts with distilled water. If thegel polymerize