Bio-rad Precision Plus Protein™ Standard Plugs User Manual

Instruction ManualPrecision Plus Protein™Standard Plugs, UnstainedCatalog #161-0378Product shipped at room temperature.Store at 4ºC upon arrival.41176

8Fig. 2. Break and twist off one end of the plastic plug casing.4117684C.qxp 8/20/2010 10:51 AM Page 10

9Fig. 3. Break off an individual plastic plug from the casing byplacing pressure on the flat side to break the junction.Study Fig. 3 and 4 carefully b

10Fig. 4. Complete the break by twisting off the individual plasticplug casing.4117684C.qxp 8/20/2010 10:52 AM Page 12

11Fig. 5. Snap off the attached spatula.4117684C.qxp 8/20/2010 10:52 AM Page 13

12Fig. 6. Use spatula to push plug out of the plastic casing.4117684C.qxp 8/20/2010 10:52 AM Page 14

13Fig. 7. Use the spatula to slide the plug outward and on to thetop plate of your gel.4117684C.qxp 8/20/2010 10:52 AM Page 15

Fig. 8. Slide plug all the way down the bottom of the well nextto IPG strip, then add agarose overlay.Coomassie is a trademark of Imperial Chemical In

Products for 2-D Electrophoresis, Proteomics Catalog # Description161-1511 PROTEAN Plus Tris-HCl Gel, 12%, 24 cm IPG well161-0787 Bio-Safe Coomassie S

1Representativelot of PrecisionPlus Protein standard plugon a 4–20% Tris-HClSDS-PAGE gelkD375015025010075102520154117684C.qxp 8/20/2010 10:51 AM Pa

2Precision Plus Protein Unstained Standard Plugs allow you to easily, quickly,and cleanly load molecular weight standardson any gel. The newest additi

3Product DescriptionPrecision Plus Protein standard plugs contain tenrecombinant protein bands of 10 kD, 15 kD, 20 kD,25 kD, 37 kD, 50 kD, 75 kD, 100

4SpecificationsCatalog # 161-0378Number of applications 24 plugsProtein quantity 250 kD band, 540 ng; 150 kD band,315 ng; 100 kD band, 225 ng; 75 kD b

5InstructionsPrecision Plus Protein Standard Plugs are ready-to-use 2-D electrophoresis standards. When ready touse Precision Plus Protein standard pl

6Flat side of plastic plug casingRecommended Loading Volumes for Large Gel SystemsLoad PAGE ApplicationFull plug Coomassie Blue G-250 or R-250 stainin

7Fig. 1. Flat side of plastic casing with 12 Precision Plus Proteinstandard plugs, unstained.4117684C.qxp 8/20/2010 10:51 AM Page 9