Bio-rad Fluorescent Assay Kits User Manual
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Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 945474100128 Rev AFluorAce™ ß-galactosidaseReporter Assay KitInstruction ManualCatalog Numbe
Section 1 Introduction ...1Section 2 Kit Components ...
Section 1Introductionß-galactosidase from E. coli, encoded by the lac Z gene, is one of themost versatile reporters for gene expression studies. In ad
Section 4Experimental ProceduresThe FluorAce ß-galactosidase Reporter Assay Kit has been designedfor use with any fluorometer system. The following se
MUG and 42.3 µl of 14.2 M ß-mercaptoethanol to 50 ml of 1xReaction Buffer.3. Add 500 µl assay buffer prepared above to each 1.5 ml microfugetube.4. A
Fig. 1. Example plot of standard curve and linear equation.4. To calculate the concentration of 4MU released, determine the leastsquares regression e
2. Sambrook, J., Fritsch, E. F., and Maniatis, T., Molecular Cloning: ALaboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press,Cold
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