BIO RAD Bio-Plex Software® Upgrades and Conversions manuals

Owner’s manuals and user’s guides for Accessories for water BIO RAD Bio-Plex Software® Upgrades and Conversions.
We providing 1 pdf manuals BIO RAD Bio-Plex Software® Upgrades and Conversions for download free by document types: User Manual

Bio rad Bio-Plex Software® Upgrades and Conversions User Manual (260 pages)

Brand: Bio-rad | Category: Accessories for water | Size: 8.24 MB |

English

Table of contents

Bio-Plex Manager

1

Software 6.1

1

Bio-Plex Manager™

3

Software 6.1 User Guide

3

Table of Contents

5

1 Bio-Plex Suspension

13

Components

14

Advantages

15

For More Information

16

Bio-Rad Technical Support

16

2 Bio-Plex Manager™

17

Software Licenses

18

General Workflow

19

Quick Guide

20

Types of Files

21

Key Software Features

21

3 Software Installation

23

Required Screen Resolution

24

Installing the Software

25

Uninstalling

26

4 Starting the System

27

Microplate Platform

28

Starting Bio-Plex Manager

29

Menu and Toolbars

31

Status Bar

31

Sample Needle Adjustment

33

Needle height

34

5 Controlling the System

37

Bio-Plex MCV Plate IV

38

Start Up

39

Calibration

41

Setting Up the Calibration

45

Performing the Calibration

46

Logging the Calibration

46

Start Up and Calibrate

49

Wash Between Plates

50

Remove Air Bubbles

51

Unclog

52

Validation

53

Setting Up a Validation Run

54

Control Number Selection

54

Validation Type Selection

55

Performing a Validation Run

56

Validation Results

56

Validation Log

57

Platform Heater

60

Instrument Information

61

Eject/Retract Plate

61

Shut Down

63

Instrument Operations Log

63

6 Preparing Protocols

65

Saving Protocols

66

Protocol Files

67

Multi-Assay Protocols

68

Multi-Assay

69

Step 1. Describe Protocol

71

Step 2. Select Analytes

71

SELECTING ANALYTES

72

IMPORTING A PANEL OF ANALYTES

75

EDITING A PANEL OF ANALYTES

78

COMBINING PANELS OF ANALYTES

79

Step 3. Format Plate

80

PLATE FORMATTING

81

DEFINING UNKNOWN SAMPLE WELLS

81

AUTOFILLING WELL NUMBERS

82

DEFINING A REPLICATE GROUP

83

AUTOFILLING REPLICATE GROUPS

83

CHANGING A REPLICATE GROUP

84

RESEQUENCING WELL NUMBERS

84

DEFINING STANDARD WELLS

84

DEFINING CONTROL WELLS

85

DEFINING BLANK WELLS

86

Plate Groupings

87

DEFINING A GROUP

88

CONTEXT MENU

89

DEFINING THE REFERENCE

89

PRINTING THE PLATE FORMAT

89

Standard Lots

91

MANAGING STANDARD LOTS

93

DELETING STANDARDS

98

SELECTING EXTERNAL STANDARDS

99

Protocol Window

101

ENTERING CONCENTRATIONS

103

(FOR CURRENT STANDARDS ONLY)

103

CONCENTRATION UNITS

105

REGRESSION METHODS

105

COEFFICIENT B

106

LOGISTIC WEIGHTING

106

COEFFICIENT A

106

RECOVERY PERCENTAGE RANGE

107

7 Running Protocols

111

Run Protocol Window

112

Bead Count

113

Sample Timeout

113

Reservoir Functions

114

Skip Functions

115

Standard Curve Optimization

116

Advanced Run Settings

116

Sample Size

117

Save Options

117

Sampling Errors

117

Plate Loading Guidelines

119

Running the Protocol

120

Manually Stopping a Reading

122

Histogram and Bead Map

123

Histogram

124

SELECTING THE CHANNEL TYPE

125

SCALING THE HISTOGRAM DATA

126

Bead Map

127

Bead Map Display Options

129

DENSITY FILTERING

130

LOGARITHMIC/LINEAR DISPLAY

130

Raw Data Table

131

Bead Statistics

132

Sampling Error Codes

132

Raw Data Display Options

133

Set Number Format

134

Other Table Formatting

134

Copying the Raw Data

134

Rerun/Recovery Mode

135

8 Using the

137

Background on 21 CFR Part 11

138

Standard Mode vs. Secure Mode

138

Security Edition

139

User Level Restrictions

140

User Information

140

User Authentication

141

Electronic Records

142

Operations Logs

143

Samples in a Protocol

147

Audit Trail

149

Viewing the Audit Trail

151

Protected Directories

152

Locking Bio-Plex Manager

153

9 Analyzing the Results

155

Opening a Results File

156

Saving a Results File

156

Results Files

157

Results Window

158

Custom Reports

159

Raw Data

162

Exporting the Raw Data

163

Raw Data Table Error Codes

164

Printing the Raw Data

164

Report Table

165

Display Options

166

PREDEFINED REPORT SCHEMES

167

ORGANIZING BY TYPE OR GROUP

168

EXPAND REPLICATE INFO

168

SETTING NUMBER FORMATS

169

Obs/Exp * 100 Column

172

Concentration in Range Column

172

Showing/Hiding Outliers

173

Resizing the Columns

174

Context Menus

174

Sorting Report Table Data

175

Copying the Report Table

175

Printing the Report Table

175

Exporting the Report Table

175

Export Format

176

Export a Subset of the Data

177

Export Preferences

178

Use Multiple Worksheets

179

Exclude Standard Curves

179

Exclude Table Error Codes

179

Standard Curve

180

Regression Methods

182

LOGISTIC-5PL

183

LOGISTIC-4PL

183

CUBIC SPLINE

184

POINT-TO-POINT

184

Copying the Standard Curve

185

Exporting the Standard Curve

185

Printing the Standard Curve

186

Graphing Function

187

Choosing Graph Options

189

Adding New Graphs

194

Editing an Existing Graph

195

Deleting a Graph

196

Adjusting Your Graphs

197

Printing Graphs

197

Document Export Options

198

Bio-Plex XML Export

199

XML SCHEMA FILE

200

Output CSV File Format

201

ANALYTE SORT ORDER

202

Using Stylesheets

203

Command Line Export

204

Exporting the Document

205

10 Standard Curve

211

Background

212

Determining Outliers

213

COMMENTS

215

Logistic Weighting

216

11 Data Normalization

219

Assigning a Control Sample

221

Report Table Options

222

Normalization Definitions

224

Tab l e 1

226

Troubleshooting

227

Appendix

231

Normalization Formulas

235

Norm Ratio

236

Norm Ratio’

236

Norm Ratio’’

236

Basic Concepts

239

Fluidics

240

Excitation

240

Microsphere Handling

241

CENTRIFUGATION

242

Software Warranty

244

Reporting Problems to Bio-Rad

245

Report Table Error Codes

246

Glossary

247

Ter m D e f in i t ion

248

Numerics

253

Life Science

260

Bio-Rad

260

Laboratories, Inc

260

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