Bio-rad SmartSpec Plus Spectrophotometer User Manual download pdf (Page 2)

Table of Contents

Page

Section 1 General Safety Information .....................................................................1

1.1 Caution Symbol......................................................................................................1

1.2 Precautions.............................................................................................................1

Section 2 Packing List ..............................................................................................1

Section 3 Installation ................................................................................................2

3.1 Environmental Requirements .................................................................................2

3.2 Power On and Initial Display .................................................................................2

Section 4 Introduction ..............................................................................................3

4.1 Identification of System Components.....................................................................3

4.2 Setup ......................................................................................................................7

4.3 Serial Port Functions ............................................................................................10

4.4 PC Serial Connection Instructions........................................................................11

4.5 Key Pad Descriptions ...........................................................................................13

Section 5 Quick Guide............................................................................................15

Section 6 Operating Instructions ...........................................................................17

Section 7 Examples of Assays.................................................................................25

7.1 DNA/RNA ...........................................................................................................25

7.2 Protein Assay .......................................................................................................31

7.3 Spectral Scanning.................................................................................................44

7.4 Kinetics ................................................................................................................46

7.5 OD600..................................................................................................................47

7.6 Lambda (λ) ..........................................................................................................48

Section 8 Troubleshooting ......................................................................................49

Section 9 Maintenance............................................................................................50

9.1 Cleaning Up a Fluid Spill .....................................................................................50

9.2 Cleaning Quartz Cuvettes.....................................................................................51

9.3 Changing Thermal Printer Paper ..........................................................................51

Section 10 Specifications ..........................................................................................52

Section 11 System, Accessories, and Reagents for SmartSpec

Spectrophotometer .................................................................................53

11.1 Product Information .............................................................................................53

11.2 Bio-Rad Protein Assays........................................................................................53

11.3 Cuvette Selection Guide .......................................................................................54

11.4 Selecting the Appropriate Cuvette........................................................................54

For technical service call your local Bio-Rad office. In the U.S., call 1-800-4BIORAD

(1-800-424-6723).

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Bio-rad SmartSpec Plus Spectrophotometer User Manual Page 2